Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan
Autor: | Shazia Iqbal, Ali Akbar, Wajeeha Naeem, Muhammad Sabieh Anwar, Anila Bahadur, Sareeen Fatima, Faiza Liaqat, Gul Makai, Muhammad Sahfee, Abdul Samad, Adnan Khan, S. A. Khan, Haleema Sadia |
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Rok vydání: | 2021 |
Předmět: |
Imipenem
Klebsiella Nalidixic acid Klebsiella pneumoniae Virulence Microbial Sensitivity Tests Dermatology Drug resistance beta-Lactamases antibiotics Microbiology 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine opportunistic pathogens nosocomial infections medicine Humans pathogenicity 030212 general & internal medicine Cross Infection biology business.industry Original Articles biology.organism_classification Anti-Bacterial Agents Ciprofloxacin Amikacin antimicrobial Original Article Surgery business medicine.drug |
Zdroj: | International Wound Journal |
ISSN: | 1742-481X 1742-4801 |
Popis: | Klebsiella pneumoniae is an important pathogen causing hospital‐acquired infections in human beings. Samples from suspected patients of K pneumoniae associated with respiratory and urinary tract infections were collected at Bolan Medical Complex, Quetta, Balochistan. Clinical samples (n = 107) of urine and sputum were collected and processed for K pneumoniae isolation using selective culture media. Initially, 30 of 107 isolates resembling Klebsiella spp. were processed for biochemical profiling and molecular detection using gyrase A (gyrA) gene for conformation. The K pneumoniae isolates were analysed for the presence of drug resistance and virulence genes in their genomes. The 21 of 107 (19.6%) isolates were finally confirmed as K pneumoniae pathogens. An antibiogram study conducted against 17 different antibiotics showed that a majority of the isolates are multidrug resistant. All the isolates (100%) were resistant to amoxicillin, cefixime, amoxicillin‐clavulanic acid, cefotaxime, and ceftriaxone followed by tetracycline (95.2%), ciprofloxacin and gentamicin (76.2%), sulphamethoxazol (66.7%), nalidixic acid (61.9%), norfloxacine (42.9%), piperacillin‐tazobactam (23.8%), cefoperazone‐sulbactam (19%), and cefotaxime‐clavulanic acid (33.3%), whereas all the isolates showed sensitivity to amikacin, chloramphenicol, and imipenem. The presence of tetracycline, sulphamethoxazol‐resistant genes, and extended‐spectrum beta‐lactamase was reconfirmed using different specific genes. The presence of virulence genes fimH1 and EntB responsible for adherence and enterobactin production was confirmed in the isolates. The high virulence and drug resistance potential of these Klebsiella isolates are of high public health concern. Multidrug resistance and virulence potential in K. pneumoniae are converting these nosocomial pathogens into superbugs and making its management harder. |
Databáze: | OpenAIRE |
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