Highly prevalent emmSTG840.0 and emmSTC839.0 types of erythromycin non-susceptible group G Streptococcus isolated from bacteremia in southern Taiwan

Autor: Jiunn Jong Wu, Yuen Chi Chan, Chien-Shun Chiou, Po Xing Zheng, Cheng Lu Hsieh, Chuan Chiang-Ni
Rok vydání: 2017
Předmět:
0301 basic medicine
Microbiology (medical)
Southern taiwan
030106 microbiology
Taiwan
lcsh:QR1-502
Erythromycin
Bacteremia
Biology
lcsh:Microbiology
Microbiology
Agar dilution
law.invention
Group G streptococcus
03 medical and health sciences
Bacterial Proteins
Disk Diffusion Antimicrobial Tests
law
Streptococcal Infections
Drug Resistance
Bacterial
medicine
Humans
Immunology and Allergy
Polymerase chain reaction
Gel electrophoresis
Antigens
Bacterial
General Immunology and Microbiology
Membrane Proteins
Streptococcus
Methyltransferases
General Medicine
biology.organism_classification
medicine.disease
Virology
Anti-Bacterial Agents
Infectious Diseases
Spectrometry
Mass
Matrix-Assisted Laser Desorption-Ionization
Carrier Proteins
Streptococcus dysgalactiae
Bacterial Outer Membrane Proteins
medicine.drug
Zdroj: Journal of Microbiology, Immunology and Infection, Vol 50, Iss 6, Pp 831-838 (2017)
ISSN: 1684-1182
DOI: 10.1016/j.jmii.2016.12.010
Popis: Background/Purpose: Group G Streptococcus (GGS) infections in human have increased. Treatment relied on antibiotic therapy, including erythromycin. However, information regarding the dominant strains and erythromycin susceptibility in GGS bacteremia is limited. Methods: A total of 134 GGS were isolated from patients with bacteremia in a university hospital of southern Taiwan during 1993â2010. The erythromycin susceptibility was determined by disc diffusion and agar dilution assays. The bacterial species was determined by MALDI-TOF. The presence of erythromycin-resistant genes and emm types were determined by polymerase chain reaction and sequence. The clonal spreading was analyzed by pulsed-field gel electrophoresis with SmaI or SgrAI digestion. Results: The annual erythromycin non-susceptible rate varied, with an average of 40.3%. All erythromycin non-susceptible strains belonged to the Streptococcus dysgalactiae. No erythromycin non-susceptible strains belong to the anginosus group. The most prevalent erythromycin-resistant gene was mefA (57.4%), followed by ermB (37%), and ermA (3.7%). The N terminal hyper variable region of emm was sequenced to determine the emm type, and only S. dysgalactiae had the emm gene. The most prevalent emm types were emmSTG840.0 (17.2%), emmSTG485.0 (10.4%), and emmSTC839.0 (9.0%). 73% and 47% of the strains with only mefA and ermB belonged to emmSTG840.0 and emmSTC839.0 types, respectively. Pulsed-field gel electrophoresis showed that different clones of emmSTG840.0 and emmSTC839.0 strains were spread in this region during the 18 years of surveillance. Conclusion: Our data indicate that there were dominant emm types with erythromycin non-susceptibility in S. dysgalactiae isolated from bacteremia in Taiwan, and thus constant surveillance is warranted. Keywords: emm, Epidemiology, Erythromycin, Group G Streptococcus
Databáze: OpenAIRE
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