LC determination of oxcarbazepine and its active metabolite in human serum
| Autor: | Pascal Odou, H. Levert, H Robert |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Phenytoin
medicine.medical_treatment Metabolite Clinical Biochemistry Oxcarbazepine Pharmaceutical Science Pharmacology Analytical Chemistry chemistry.chemical_compound Pharmacokinetics Drug Discovery medicine Humans Biotransformation Chromatography High Pressure Liquid Spectroscopy Active metabolite Models Statistical Chromatography Chemistry Reproducibility of Results Carbamazepine Anticonvulsant Calibration Regression Analysis Anticonvulsants Phenobarbital medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 28:517-525 |
| ISSN: | 0731-7085 |
| Popis: | Twenty-five percent of epileptic patients present refractory seizures to current frontline antiepileptic drugs, needing new treatments and leading to the introduction of several new AEDs, among which is oxcarbazepine (Trileptal ® ). This 10-ketoanalogue of carbamazepine seems to be a weaker inducer of cytochrome P450 3A4. However, pharmacokinetic interactions with clinical significance have already been reported, before the marketing of Trileptal ® in France. The aim of this study was to develop and validate a HPLC method allowing simultaneous dosage of oxcarbazepine, 10-hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin. After plasma defecation by acetonitrile, dosage was obtained by analysis of the supernatants on a C 18 reversed-phase column coupled with UV detection (240 nm). The statistical validation was performed according to the recommendations of a European technical commission. This method seems to provide a quite good selectivity from the psychotropic therapeutics, which is commonly coprescribed with AEDs. Linearity was established for the whole concentration range, whatever the compound. Quantization limits of oxcarbazepine, 10-hydroxycarbamazepine, epoxycarbamazepine, carbamazepine, phenobarbital and phenytoin are 0.58, 3.5, 2.35, 0.66, 1.02 and 3.13 μg/ml, respectively, and absolute recoveries are 105.15, 84.76, 94.45, 96.52, 98.62 and 95.08%, respectively. |
| Databáze: | OpenAIRE |
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