Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF)
| Autor: | Toshikazu Fukui, Joost J. Oppenheim, Yasuji Furutani, Mitsue Notake, Masaaki Yamada, Kouji Matsushima, Yoshihiro Oyamada, Hideki Nomura, Christian G. Larsen |
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| Rok vydání: | 1989 |
| Předmět: |
Signal peptide
Molecular Sequence Data Biophysics Protein Sorting Signals Biology Biochemistry Homology (biology) Biological Factors Sequence Homology Nucleic Acid Complementary DNA Tumor Cells Cultured medicine Humans Amino Acid Sequence Cyanogen Bromide RNA Messenger Cloning Molecular Protein Precursors Codon Molecular Biology Peptide sequence Macrophage inflammatory protein Chemokine CCL2 Base Sequence Chemotactic Factors Monocyte Nucleic acid sequence Protein primary structure Nucleic Acid Hybridization DNA Cell Biology Macrophage Activation Molecular biology Peptide Fragments Chemotaxis Leukocyte medicine.anatomical_structure Electrophoresis Polyacrylamide Gel Oligonucleotide Probes |
| Zdroj: | Biochemical and Biophysical Research Communications. 159:249-255 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(89)92430-3 |
| Popis: | cDNA clones having a nucleotide sequence encoding a human monocyte chemotactic and activating factor (MCAF) were isolated and sequenced. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. The amino acid sequence of MCAF showed 25-55% homology with other members of an inducible cytokine family, including macrophage inflammatory protein and some putative polypeptide mediators known as JE, LD78, RANTES and TCA-3. This suggests that MCAF is a member of family of factors involved in immune and inflammatory responses. |
| Databáze: | OpenAIRE |
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