Comparative Analysis of Cytotoxic T Lymphocytes in Lymph Nodes and Peripheral Blood of Simian Immunodeficiency Virus-Infected Rhesus Monkeys
| Autor: | Carol I. Lord, Jörn E. Schmitz, William A. Charini, Norman L. Letvin, Marcelo J. Kuroda, Christine E. Nickerson, Meryl A. Forman |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Immunology
Simian Acquired Immunodeficiency Syndrome Epitopes T-Lymphocyte Gene Products gag chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes medicine.disease_cause Major histocompatibility complex Microbiology Immunophenotyping Virology medicine Lymph node stromal cell Animals Cytotoxic T cell Lymph node biology Immunodominant Epitopes Histocompatibility Antigens Class I hemic and immune systems Simian immunodeficiency virus Macaca mulatta CTL medicine.anatomical_structure Insect Science Leukocytes Mononuclear biology.protein Pathogenesis and Immunity Simian Immunodeficiency Virus Lymph Nodes Lymph CD8 T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Virology. 73:1573-1579 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.73.2.1573-1579.1999 |
| Popis: | Most studies of human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocytes (CTL) have been confined to the evaluation of these effector cells in the peripheral blood. What has not been clear is the extent to which CTL activity in the blood actually reflects this effector cell function in the lymph nodes, the major sites of HIV-1 replication. To determine the concordance between CTL activity in lymph nodes and peripheral blood lymphocytes (PBL), CTL specific for simian immunodeficiency virus of macaques (SIVmac) have been characterized in lymph nodes of infected, genetically selected rhesus monkeys by using both Gag peptide-specific functional CTL assays and tetrameric peptide-major histocompatibility complex (MHC) class I molecule complex staining techniques. In studies of six chronically SIVmac-infected rhesus monkeys, Gag epitope-specific functional lytic activity and specific tetrameric peptide-MHC class I staining were readily demonstrated in lymph node T lymphocytes. Although the numbers of tetramer-binding cells in some animals differed from those documented in their PBL, the numbers of tetramer-binding cells from these two different compartments were not statistically different. Phenotypic characterization of the tetramer-binding CD8 + lymph node T lymphocytes of the infected monkeys demonstrated a high level of expression of the activation-associated adhesion molecules CD11a and CD49d, the Fas molecule CD95, and MHC class II-DR. These studies documented a low expression of the naive T-cell marker CD45RA and the adhesion molecule CD62L. This phenotypic profile of the tetramer-binding lymph node CD8 + T cells was similar to that of tetramer-binding CD8 + T cells from PBL. These observations suggest that characterization of AIDS virus-specific CTL activity by sampling of cells in the peripheral blood should provide a reasonable estimation of CTL in an individual’s secondary lymphoid tissue. |
| Databáze: | OpenAIRE |
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