Burkholderia puraquae sp. nov., a novel species of the Burkholderia cepacia complex isolated from hospital settings and agricultural soils
| Autor: | Carla C. C. R. de Carvalho, Marisa Bettiol, Sergio Lejona, Silvina Benetti, Alejandra Bosch, Angeles Zorreguieta, Mariana Leguizamon, Cecilia Beatriz Fígoli, Claudia Prieto, Cecilia Vescina, Osvaldo Yantorno, Florencia Alvarez, Sílvia A. Sousa, Walter Omar Draghi, Patricia Montanaro, Pablo Martina, Peter Lasch, Julian Alberto Ferreras, Antonio Lagares, Juliana Palau, Jorge H. Leitão, Maren Stämmler |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial Cystic Fibrosis Sequence analysis Biología 030106 microbiology Microbiology Aesculin 03 medical and health sciences chemistry.chemical_compound Burkholderia genus Species Specificity RNA Ribosomal 16S Humans Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Ciencias Exactas Base Composition biology Burkholderia cepacia complex Fatty Acids Sputum Agriculture General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques 030104 developmental biology Burkholderia Burkholderia puraquae sp. nov chemistry Genes Bacterial Multilocus sequence typing Soil microbiology Bacteria Multilocus Sequence Typing |
| Zdroj: | SEDICI (UNLP) Universidad Nacional de La Plata instacron:UNLP |
| Popis: | Bacteria from the Burkholderia cepacia complex (Bcc) are capable of causing severe infections in patients with cystic fibrosis (CF). These opportunistic pathogens are also widely distributed in natural and man-made environments. After a 12-year epidemiological surveillance involving Bcc bacteria from respiratory secretions of Argentinean patients with CF and from hospital settings, we found six isolates of the Bcc with a concatenated species-specific allele sequence that differed by more than 3% from those of the Bcc with validly published names. According to the multilocus sequence analysis (MLSA), these isolates clustered with the agricultural soil strain, Burkholderia sp. PBP 78, which was already deposited in the PubMLST database. The isolates were examined using a polyphasic approach, which included 16S rRNA, recA, Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), DNA base composition, average nucleotide identities (ANIs), fatty acid profiles, and biochemical characterizations. The results of the present study demonstrate that the seven isolates represent a single novel species within the Bcc, for which the name Burkholderia puraquae sp. nov. is proposed. Burkholderia puraquae sp. nov. CAMPA 1040T (=LMG 29660T=DSM 103137T) was designated the type strain of the novel species, which can be differentiated from other species of the Bcc mainly from recA gene sequence analysis, MLSA, ANIb, MALDI-TOF MS analysis, and some biochemical tests, including the ability to grow at 42 ºC, aesculin hydrolysis, and lysine decarboxylase and β-galactosidase activities. Facultad de Ciencias Exactas Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Biotecnologia y Biologia Molecular |
| Databáze: | OpenAIRE |
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