Metabolic profile of human coelomic fluid
| Autor: | Charalambos Fotakis, Georgios Theodoridis, K Chatzimeletiou, Leandros Lazaros, Michael Witting, Anastasia Chrysovalantou Chatziioannou, Franco Moritz, G. Makrydimas, L Valianou, Christina Virgiliou, Nikolaos Raikos, Panagiotis Zoumpoulakis |
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| Rok vydání: | 2017 |
| Předmět: |
Magnetic Resonance Spectroscopy
Resolution (mass spectrometry) Clinical Biochemistry Gestational Age Biology Tandem mass spectrometry 01 natural sciences Gas Chromatography-Mass Spectrometry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Metabolomics Tandem Mass Spectrometry Metabolome Humans General Pharmacology Toxicology and Pharmaceutics 030219 obstetrics & reproductive medicine Chromatography Hydrophilic interaction chromatography 010401 analytical chemistry Coelomic Fluid Coelomic Fluid Cells General Medicine Embryo Mammalian Body Fluids 0104 chemical sciences Medical Laboratory Technology Gestational Sac Female Gas chromatography–mass spectrometry Ultra high performance Metabolic profile Chromatography Liquid |
| Zdroj: | Bioanalysis 9, 37-51 (2017) |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Aim: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. Methodology: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC–MS/MS method was applied. Holistic analysis performed by GC–MS, NMR spectroscopy and ion cyclotron ultra-high resolution MS (FT–ICR–MS) instrumentation. Results & Conclusions: Our observations suggest coelomic fluid and cells as promising biosamples, rich in metabolites with potential use in mammalian system biology studies. |
| Databáze: | OpenAIRE |
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