Corneal epithelial basement membrane after experimental anterior stromal puncture in guinea pigs: Immunohistochemical study
| Autor: | Shizuya Saika, Yuka Okada, Akira Ooshima, Yoshitaka Ohnishi, Osamu Yamanaka, Natsuko Hashizume |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Pathology medicine.medical_specialty Stromal cell Corneal Stroma Guinea Pigs Biology Wounds Nonpenetrating Basement Membrane Immunoenzyme Techniques Type IV collagen Eye Injuries Laminin Cornea medicine Animals Corneal epithelium Basement membrane Wound Healing Epithelium Corneal Antibodies Monoclonal General Medicine Epithelium Ophthalmology medicine.anatomical_structure biology.protein Female Collagen Wound healing |
| Zdroj: | Japanese Journal of Ophthalmology. 41:376-380 |
| ISSN: | 0021-5155 |
| Popis: | We investigated alterations in the immunolocalization of the components of epithelial basement membrane (BM), type IV collagen, and laminin in guinea pigs subjected to anterior stromal puncture (ASP) of the cornea performed with a standardized needle. Localization of BM components beneath the corneal epithelium was indicated by laminin immunoreactivity. The BM was interrupted by needle punctures immediately after ASP. During healing, type IV collagen immunoreactivity was detected transiently in the BM of some of the ASP-treated corneas, but no reactivity was observed in normal epithelial BM. Development of type IV collagen immunoreactivity was probably caused by an alteration of the alpha-chains or by an unmasking of the antigenicity of this collagen type, which may be related to an increase in the adhesiveness of the epithelium following ASP. |
| Databáze: | OpenAIRE |
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