The chicken gut metagenome and the modulatory effects of plant-derived benzylisoquinoline alkaloids
| Autor: | Dan Liu, Nan-Xi Hu, Jianguo Zeng, Conghui Liu, Dazhi Tang, Hengchao Wang, Wei Fan, Jia-Lu Huang, Lijuan Yin, Li Lin, Jingyang Yuan, Xiubin Liu, Xi He, Liu Hua, Qian Lin, Peng Huang, Yan Zhang, Long Yun, Kangpeng Xiao, Xiaogang Su, Yuwei Ren, Bo Liu, Hua-Liang Cao, Fan Jiang, Zhixing Qing, Yuming Guo, Sanwen Huang, Yi-Song Liu, Cheng Zeng |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) animal structures Ranunculales 030106 microbiology Gut flora Microbiology Benzylisoquinolines digestive system lcsh:Microbial ecology 03 medical and health sciences Microbial ecology Lactobacillus Animals Microbiome Growth Substances Gene Benzylisoquinoline alkaloid biology Gut metagenome Plant Extracts Probiotics Research Antibiotic Foregut biology.organism_classification Chicken Gastrointestinal Microbiome 030104 developmental biology Metagenomics lcsh:QR100-130 Chickens Growth promoter Bacteria Chlortetracycline |
| Zdroj: | Microbiome, Vol 6, Iss 1, Pp 1-17 (2018) Microbiome |
| ISSN: | 2049-2618 |
| Popis: | Background Sub-therapeutic antibiotics are widely used as growth promoters in the poultry industry; however, the resulting antibiotic resistance threatens public health. A plant-derived growth promoter, Macleaya cordata extract (MCE), with effective ingredients of benzylisoquinoline alkaloids, is a potential alternative to antibiotic growth promoters. Altered intestinal microbiota play important roles in growth promotion, but the underlying mechanism remains unknown. Results We generated 1.64 terabases of metagenomic data from 495 chicken intestinal digesta samples and constructed a comprehensive chicken gut microbial gene catalog (9.04 million genes), which is also the first gene catalog of an animal’s gut microbiome that covers all intestinal compartments. Then, we identified the distinctive characteristics and temporal changes in the foregut and hindgut microbiota. Next, we assessed the impact of MCE on chickens and gut microbiota. Chickens fed with MCE had improved growth performance, and major microbial changes were confined to the foregut, with the predominant role of Lactobacillus being enhanced, and the amino acids, vitamins, and secondary bile acids biosynthesis pathways being upregulated, but lacked the accumulation of antibiotic-resistance genes. In comparison, treatment with chlortetracycline similarly enriched some biosynthesis pathways of nutrients in the foregut microbiota, but elicited an increase in antibiotic-producing bacteria and antibiotic-resistance genes. Conclusion The reference gene catalog of the chicken gut microbiome is an important supplement to animal gut metagenomes. Metagenomic analysis provides insights into the growth-promoting mechanism of MCE, and underscored the importance of utilizing safe and effective growth promoters. Electronic supplementary material The online version of this article (10.1186/s40168-018-0590-5) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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