Commercial preparations of colloidal gold-antibody complexes frequently contain free active antibody
| Autor: | R Sealock, N R Kramarcy |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Histology
Anticorps monoclonal medicine.drug_class Immunoelectron microscopy Fluorescent Antibody Technique Monoclonal antibody Antibodies Mice medicine Animals Colloids biology Chemistry Antigen binding Molecular biology Immunohistochemistry Antibodies Anti-Idiotypic Rats Biochemistry Colloidal gold Gold particles Immunoglobulin G biology.protein Indicators and Reagents Gold Anatomy Antibody |
| Zdroj: | The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 39(1) |
| ISSN: | 0022-1554 |
| Popis: | Using a simple fluorescence test, we show that commercially prepared colloidal gold complexes with goat second antibodies often contain free active antibody. Because such antibodies will compete with antibody-colloidal gold particles for antigen binding sites, labeling intensity at the ultrastructural level must necessarily be submaximal to an unknown degree with such preparations. A survey of five preparations suggests that the problem may be widespread. We recommend that a test of the sort described be incorporated routinely into protocols with all colloidal gold products. |
| Databáze: | OpenAIRE |
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