Cancer Immunomics: From Serological Proteome Analysis to Multiple Affinity Protein Profiling
| Autor: | Julie Hardouin, Raymonde Joubert-Caron, Loïk Sylvius, Jean-Paul Lasserre, Michel Caron |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Proteomics
Proteome Colorectal cancer General Neuroscience Autoantibody Cancer Computational biology Biology medicine.disease Molecular biology General Biochemistry Genetics and Molecular Biology Immunomics History and Philosophy of Science Affinity chromatography Antigen Neoplasms Biomarkers Tumor medicine Humans Antigens Autoantibodies |
| Zdroj: | Annals of the New York Academy of Sciences. 1107:223-230 |
| ISSN: | 0077-8923 |
| DOI: | 10.1196/annals.1381.024 |
| Popis: | Cancer remains one of the leading causes of death worldwide. Thus, to identify any useful biomarkers is still a need. We performed "cancer immunomics" to identify autoantibody signatures produced in response to the presence of either breast or colorectal cancer. SERological proteome analysis (SERPA) was performed by two-dimensional (2-D) electrophoresis separation, immunoblotting, image analysis, and mass spectrometry. Alternatively, to identify the antigens recognized by the autoantibodies of cancer patients, we developed an approach combining 2-D immunoaffinity chromatography, enzymatic digestion of the isolated antigens, nano flow separation of the resulting peptides, and identification: MAPPing (multiple affinity protein profiling). By these approaches we identified both proteins recognized by autoantibodies independently of a cancer status, and a limited number of proteins reacting preferentially with cancer sera. |
| Databáze: | OpenAIRE |
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