The value of circulating tumor cells with positive centromere probe 8 in the diagnosis of small pulmonary nodules

Autor: Xuerong Wang, Tong Sun, Baoan Chen, Hongling Chen, Haibo Wang, Caidong Liu
Rok vydání: 2021
Předmět:
0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Small pulmonary nodule
lcsh:RC254-282
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
FISH
CEP8
centromere probe 8
EpCAM
epithelial cancer-associated marker
medicine
SPNs
small pulmonary nodules
IA
invasive adenocarcinoma
CD45
Stage (cooking)
Liquid biopsy
Lung cancer
pTNM
pathological tumor nodal metastasis
FISH
fluorescence in situ
Original Research
Tumor marker
AFP
alpha-fetoprotein
business.industry
FR
folate receptor
CTCs
circulating tumor cells
MIA
minimally invasive adenocarcinoma
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
FDA
Food and Drug Administration
CT
computed tomography
Staining
CKs
cytokeratins
MTD
maximum tumor diameter
030104 developmental biology
ROC analysis
receiver operating characteristic analysis
Oncology
AUC
the area under the curve
NSCLC
non-small cell lung carcinoma
EpCAM
030220 oncology & carcinogenesis
Cancer cell
AIS
adenocarcinoma in situ
Histopathology
CEA
carcinoembryonic antigen
business
Zdroj: Translational Oncology, Vol 14, Iss 5, Pp 101052-(2021)
Translational Oncology
ISSN: 1936-5233
DOI: 10.1016/j.tranon.2021.101052
Popis: Highlights 5• CTCs numbers were significantly different between malignant and benign SPNs. 5• CTCs numbers could serve as a diagnostic marker for malignant SNPs. 5• The combined use of CTCs numbers and density feature of CT morphology further improve the overall screening and diagnostic effect of malignant SNPs, and pTNM. 5• Large, single, and solid SPNs has significant CTCs numbers correlated with the malignant histopathology. 5• Using CEP8 as a marker can efficiently detect CTCs in SPNs’ patients.
Circulating cancer cells (CTCs) can serve as a non-invasive liquid biopsy and provide opportunities for early cancer diagnosis and evaluation. However, the value of CTCs for diagnosis or prognosis of small pulmonary nodules (SPNs) is unclear. Fifty-three patients diagnosed with SPNs with a diameter less than 30 mm by CT examination were enrolled in the study. The CTC numbers, CT examination features, serum tumor marker concentrations, and histopathological characteristics were analyzed. Centromere probe 8 (CEP8) was used as a marker for CTC identification. The CTC numbers were significantly different in patients with malignant and benign SPNs and with early (0/Ⅰa) and advanced (Ⅰb/Ⅱ/Ⅲ) lung cancer stages. ROC analysis showed that the CTC numbers was effective on malignant SNP diagnosis. The combined use of CTCs and the density features of the nodules determined by CT further improved the overall screening, the diagnostic effectiveness for malignant SNPs, and determination of the pTNM (≤Ia vs.>Ia) stage. The CT morphology revealed that large, single, and solid SPNs were associated with significant CTC numbers and the CTC numbers were correlated with malignant histopathology. Using CEP8 as a marker resulted in detection of more CTC numbers in 22 patient samples triple stained for CEP8, EpCAM, and CKs. The CTCs determined by CEP8-positive staining could serve as potential screening and diagnostic markers for malignant SPNs.
Databáze: OpenAIRE
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