The CSRP2BP histone acetyltransferase drives smooth muscle gene expression
| Autor: | Bangyong Wu, Joachim Grötzinger, Ping Long, Yunjian Wei, Mark Mercola, Xinxing Jiang, Qi Li, Chao Liang, Fei Sun, Ralf Weiskirchen, Yanlin Ma, Jun Wang, Ankang Li, Wei Yu, Yanhong Yu, Yanxing Wei, Yuanhua Huang, Robert J. Schwartz |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Myocytes Smooth Muscle Gene Expression Biology Cell Line Histones 03 medical and health sciences Mice 0302 clinical medicine Serum response factor Coactivator Gene expression Genetics Myocyte Animals Humans Promoter Regions Genetic Cells Cultured Histone Acetyltransferases Regulation of gene expression Cell Nucleus Gene regulation Chromatin and Epigenetics Nuclear Proteins Acetylation Histone acetyltransferase Molecular biology Chromatin 3. Good health Rats 030104 developmental biology Gene Expression Regulation Myocardin Acetyltransferase embryonic structures biology.protein cardiovascular system Trans-Activators 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Nucleic Acids Research Nucleic acids research 45(6), 3046-3058 (2017). doi:10.1093/nar/gkw1227 |
| ISSN: | 1362-4962 |
| DOI: | 10.1093/nar/gkw1227 |
| Popis: | The expression of nearly all smooth muscle genes are controlled by serum response factor binding sites in their promoter regions. However, SRF alone is not sufficient for regulating smooth muscle cell development. It associates with other cardiovascular specific cofactors to regulate smooth muscle gene expression. Previously, we showed that the transcription co-factor CRP2 was a regulator of smooth muscle gene expression. Here, we report that CSRP2BP, a coactivator for CRP2, is a histone acetyltransferase and a driver of smooth muscle gene expression. CSRP2BP directly interacted with SRF, CRP2 and myocardin. CSRP2BP synergistically activated smooth muscle gene promoters in an SRF-dependent manner. A combination of SRF, GATA6 and CRP2 required CSRP2BP for robust smooth muscle gene promoter activity. Knock-down of Csrp2bp in smooth muscle cells resulted in reduced smooth muscle gene expression. We conclude that the CSRP2BP histone acetyltransferase is a coactivator for CRP2 that works synergistically with SRF and myocardin to regulate smooth muscle gene expression. |
| Databáze: | OpenAIRE |
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