Biosynthesis of riboflavin: cloning, sequencing, and expression of the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli
| Autor: | Cornelia Krieger, U Röthlisberger, R Volk, and Adelbert Bacher, Gerald Richter, H W Lahm |
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| Rok vydání: | 1992 |
| Předmět: |
Riboflavin
Molecular Sequence Data Sequence alignment Molecular cloning Biology medicine.disease_cause Microbiology Ribulosephosphates Bacterial Proteins Escherichia coli medicine Amino Acid Sequence Cloning Molecular Intramolecular Transferases Molecular Biology Peptide sequence Gene Heat-Shock Proteins Base Sequence Molecular mass Escherichia coli Proteins Nucleic acid sequence Gene Expression Regulation Bacterial Molecular biology Open reading frame Biochemistry Sugar Phosphates Carbohydrate Epimerases Sequence Alignment Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 174:4050-4056 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.174.12.4050-4056.1992 |
| Popis: | 3,4-Dihydroxy-2-butanone 4-phosphate is biosynthesized from ribulose 5-phosphate and serves as the biosynthetic precursor for the xylene ring of riboflavin. The gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase of Escherichia coli has been cloned and sequenced. The gene codes for a protein of 217 amino acid residues with a calculated molecular mass of 23,349.6 Da. The enzyme was purified to near homogeneity from a recombinant E. coli strain and had a specific activity of 1,700 nmol mg-1 h-1. The N-terminal amino acid sequence and the amino acid composition of the protein were in agreement with the deduced sequence. The molecular mass as determined by ion spray mass spectrometry was 23,351 +/- 2 Da, which is in agreement with the predicted mass. The previously reported loci htrP, "luxH-like," and ribB at 66 min of the E. coli chromosome are all identical to the gene coding for 3,4-dihydroxy-2-butanone 4-phosphate synthase, but their role had not been hitherto determined. Sequence homology indicates that gene luxH of Vibrio harveyi and the central open reading frame of the Bacillus subtilis riboflavin operon code for 3,4-dihydroxy-2-butanone 4-phosphate synthase. |
| Databáze: | OpenAIRE |
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