Isolation and characterization of an abortifacient protein, momorcochin, from root tubers of Momordica cochinchinensis (Family Cucurbitaceae)
| Autor: | W.W. Li, H.W. Yeung, N.S. Wong, Tzi Bun Ng |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Momordica cochinchinensis Size-exclusion chromatography Ion chromatography Biochemistry chemistry.chemical_compound Animals Amino Acids Immunoelectrophoresis Ammonium sulfate precipitation Glycoproteins Plant Proteins Gel electrophoresis Plants Medicinal Chromatography Abortifacient Agents biology Immune Sera Chromatography Ion Exchange biology.organism_classification Molecular Weight DEAE-Sepharose chemistry Sephadex Chromatography Gel Rabbits Cucurbitaceae |
| Zdroj: | International Journal of Peptide and Protein Research. 30:135-140 |
| ISSN: | 0367-8377 |
| DOI: | 10.1111/j.1399-3011.1987.tb03321.x |
| Popis: | A glycoprotein with a molecular weight of 32,000 as estimated by SDS-polyacrylamide gel electrophoresis, and characterized by an abundance of Asp and Glu residues and an absence of Cys residues in its amino acid analysis, was isolated from fresh root tubers of Momordica cochinchinensis using a procedure that involved acetone precipitation, ammonium sulfate precipitation, ion exchange chromatography on DEAE Sepharose CL-6B and gel filtration on Sephadex G-75. The protein was capable of inducing mid-term abortion in mice. The characteristics of this protein were compared and contrasted with those of the abortifacient proteins isolated from other plants of the Cucurbitaceae family. |
| Databáze: | OpenAIRE |
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