A novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection
| Autor: | Mette Boye, Martin Weiss Nielsen, Kirstine Klitgaard, Claus Schafer-Nielsen, Paolo Marcatili, Morten Nielsen, Thomas Sicheritz-Pontén, Tim Kåre Jensen |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Coinfection/genetics Ciencias de la Salud Disease RNASEQ Digital Dermatitis/genetics HIGH-DENSITY PEPTIDE ARRAYS Cattle Diseases/genetics RNA Ribosomal 16S RNA/chemistry High-density peptide arrays Pathogen Phylogeny Genetics RNA Ribosomal 16S/genetics Coinfection High-Throughput Nucleotide Sequencing RNAseq Host-Pathogen Interactions/genetics Digital dermatitis Integrated pipeline RNAseq Digital dermatitis High-density peptide arrays Host-Pathogen Interactions purl.org/becyt/ford/3 [https] Peptide microarray DNA microarray Research Article Biotechnology Fastidious organism Virulence Factors/genetics CIENCIAS MÉDICAS Y DE LA SALUD Virulence Factors Protein Array Analysis Cattle Diseases Immunoglobulins Biology Microbiology purl.org/becyt/ford/3.3 [https] 03 medical and health sciences Immune system Antigen medicine Bacteroidetes/classification Animals Salud Ocupacional Integrated pipeline Bacteroidetes Sequence Analysis RNA DIGITAL DERMATITIS Genetic Variation medicine.disease Immunoglobulins/genetics INTEGRATED PIPELINE Disease Models Animal 030104 developmental biology RNA Cattle Transcriptome |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Marcatili, P, Weiss Nielsen, M, Sicheritz-Pontén, T, Jensen, T K, Schafer-Nielsen, C, Boye, M, Nielsen, M & Schou, K K 2016, ' A novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection ', BMC Genomics, vol. 17, no. 1, 987 . https://doi.org/10.1186/s12864-016-3341-7 BMC Genomics Marcatili, P, Nielsen, M W, Sicheritz-Ponten, T, Jensen, T K, Schafer-Nielsen, C, Boye, M, Nielsen, M & Klitgaard, K 2016, ' A novel approach to probe host-pathogen interactions of bovine digital dermatitis, a model of a complex polymicrobial infection ', BMC Genomics, vol. 17, 987 . https://doi.org/10.1186/s12864-016-3341-7 |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-016-3341-7 |
| Popis: | Background: Polymicrobial infections represent a great challenge for the clarification of disease etiology and the development of comprehensive diagnostic or therapeutic tools, particularly for fastidious and difficult-to-cultivate bacteria. Using bovine digital dermatitis (DD) as a disease model, we introduce a novel strategy to study the pathogenesis of complex infections. Results: The strategy combines meta-transcriptomics with high-density peptide-microarray technology to screen for in vivo-expressed microbial genes and the host antibody response at the site of infection. Bacterial expression patterns supported the assumption that treponemes were the major DD pathogens but also indicated the active involvement of other phyla (primarily Bacteroidetes). Bacterial genes involved in chemotaxis, flagellar synthesis and protection against oxidative and acidic stress were among the major factors defining the disease. Conclusions: The extraordinary diversity observed in bacterial expression, antigens and host antibody responses between individual cows pointed toward microbial variability as a hallmark of DD. Persistence of infection and DD reinfection in the same individual is common; thus, high microbial diversity may undermine the host's capacity to mount an efficient immune response and maintain immunological memory towards DD. The common antigenic markers identified here using a high-density peptide microarray address this issue and may be useful for future preventive measures against DD. Fil: Marcatili, Paolo. Technical University of Denmark; Dinamarca Fil: Nielsen, Martin W.. Technical University of Denmark; Dinamarca Fil: Sicheritz Ponten, Thomas. Technical University of Denmark; Dinamarca Fil: Jensen, Tim K.. Technical University of Denmark; Dinamarca Fil: Schafer Nielsen, Claus. Schafer-N ApS; Dinamarca Fil: Boye, Mette. Hospital of Southern Jutland; Dinamarca Fil: Nielsen, Morten. Technical University of Denmark; Dinamarca. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Klitgaard, Kirstine. Technical University of Denmark; Dinamarca |
| Databáze: | OpenAIRE |
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