Dibutyltin activates MAP kinases in human natural killer cells, in vitro
| Autor: | Erica Taylor Isom, Abraham B. Abraha, Margaret M. Whalen, Sabah O. Odman-Ghazi |
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| Rok vydání: | 2010 |
| Předmět: |
Cytotoxicity
Immunologic Male Cell Survival MAP Kinase Signaling System Health Toxicology and Mutagenesis Blotting Western Biology Mitogen-activated protein kinase kinase Toxicology Article Organotin Compounds Humans Phosphorylation Protein kinase A Cells Cultured Mitogen-Activated Protein Kinase Kinases chemistry.chemical_classification Kinase Cell Biology Protein-Tyrosine Kinases In vitro Cell biology Killer Cells Natural Enzyme chemistry Environmental Pollutants Female Mitogen-Activated Protein Kinases Signal transduction Function (biology) |
| Zdroj: | Cell Biology and Toxicology. 26:469-479 |
| ISSN: | 1573-6822 0742-2091 |
| DOI: | 10.1007/s10565-010-9157-3 |
| Popis: | Previous studies have shown that dibutyltin (DBT) interferes with the function of human natural killer (NK) cells, diminishing their capacity to destroy tumor cells, in vitro. DBT is a widespread environmental contaminant and has been found in human blood. As NK cells are our primary immune defense against tumor cells, it is important to understand the mechanism by which DBT interferes with their function. The current study examines the effects of DBT exposures on key enzymes in the signaling pathway that regulates NK responsiveness to tumor cells. These include several protein tyrosine kinases (PTKs), mitogen activated protein kinases (MAPKs) and mitogen activated protein kinase kinases (MAP2Ks). The results showed that in vitro exposures of NK cells to DBT had no effect on PTKs. However, exposures to DBT for as little as 10 min were able to increase the phosphorylation (activation) of the MAPKs. The DBT-induced activations of these MAPKs, appears to be due to DBT-induced activations of the immediate upstream activators of the MAPKs, MAP2Ks. The results suggest that DBT-interference with the MAPK signaling pathway is a consequence of DBT exposures, which could account for DBT-induced decreases in NK function. |
| Databáze: | OpenAIRE |
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