Effect of 60 Hz electromagnetic fields on the activity of hsp70 promoter: an in vivo study
| Autor: | Heredia-Rojas Ja, Martha Santoyo-Stephano, Castañeda-Garza E, Rodríguez-De la Fuente Ao, Reyes Tamez-Guerra, Juan Manuel Alcocer-González, Laura E. Rodríguez-Flores, Cristina Rodríguez-Padilla |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
PEMF
pulsed EMF animal structures muscle S7 Biology Bioinformatics S3 Plasmid In vivo pEMF EMF plasmid Gene expression EMF electromagnetic field Luciferase Luciferase Gene Gene Quadriceps Muscles RLU relative light units Cell Biology luciferase ETS E twenty-six Molecular biology MCP-1 monocyte chemotactic protein-1 Hsp70 in vivo hsp70 heat-shock protein 70 gene expression iNOS inducible nitric oxide synthase Hsp70 promoter NF-κB nuclear factor κB 60 Hz electromagnetic fields Research Article |
| Zdroj: | Cell Biology International Reports |
| ISSN: | 2041-5346 |
| Popis: | Exposure to EMFs (electromagnetic fields) results in a number of important biological changes, including modification of genetic expression. We have investigated the effect of 60 Hz sinusoidal EMFs at a magnetic flux density of 80 μT on the expression of the luciferase gene contained in a plasmid labelled as pEMF (EMF plasmid). This gene construct contains the specific sequences for the induction of hsp70 (heat-shock protein 70) expression by EMFs, as well as the reporter for the luciferase gene. The pEMF vector was electrotransferred into quadriceps muscles of BALB/c mice that were later exposed to EMFs. Increased luciferase expression was observed in mice exposed to EMFs 2 h daily for 7 days compared with controls (P |
| Databáze: | OpenAIRE |
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