Chemical-genomic dissection of the CTD code
| Autor: | Juan B. Rodríguez-Molina, Brent E. White, Sohail Akhtar, David W. Zhang, Kevan M. Shokat, Aseem Z. Ansari, Xin Li, Rob D. Chapman, Joshua R. Tietjen, Sunduz Keles, Martin Heidemann, Dirk Eick |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
RNA
Untranslated Transcription Genetic Biophysics RNA polymerase II Biology Protein Serine-Threonine Kinases Medical and Health Sciences Article Substrate Specificity 03 medical and health sciences Open Reading Frames 0302 clinical medicine Genetic Structural Biology Transcription (biology) Genetics Phosphorylation Molecular Biology RNA polymerase II holoenzyme 030304 developmental biology 0303 health sciences Genome General transcription factor Human Genome Untranslated Biological Sciences Protein-Serine-Threonine Kinases Multigene Family Chemical Sciences biology.protein RNA Transcription factor II F CTD Transcription factor II E RNA Polymerase II Transcription factor II D Transcription 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Nature structural & molecular biology Nature structural & molecular biology, vol 17, iss 9 |
| ISSN: | 1545-9985 1545-9993 |
| Popis: | Summary Sequential modifications of the RNA polymerase II (Pol II) carboxyl-terminal domain (CTD) coordinate the stage-specific association and release of cellular machines during transcription. Here we examine the genome-wide distributions of the “early” (phospho-serine 5), “mid” (phospho-serine 7) and “late” (phospho-serine 2) CTD marks. We identify gene-class specific patterns and find widespread co-occurrence of the CTD marks. Contrary to its role in 3’ processing of non-coding RNA, the Ser7-P marks are placed early and retained until transcription termination at all Pol II-dependent genes. Chemical-genomic analysis reveals that the promoter-distal Ser7-P marks are not remnants of early phosphorylation, but are placed anew by the CTD kinase Bur1. Consistent with the ability of Bur1 to facilitate transcription elongation and suppress cryptic transcription, high levels of Ser7-P are observed at highly transcribed genes. We propose that Ser7-P could facilitate elongation and suppress cryptic transcription. |
| Databáze: | OpenAIRE |
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