Estrogen alters hippocampal dendritic spine shape and enhances synaptic protein immunoreactivity and spatial memory in female mice
| Autor: | John C. Dunlop, Marisa Gordon, Bruce S. McEwen, Wayne G. Brake, Paul Greengard, Russell D. Romeo, Ana Maria Magarinos, Victoria N. Luine, Chenjian Li, Patrick B. Allen, Rodica Buzescu |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Dendritic spine
Ovariectomy Immunocytochemistry Presynaptic Terminals Hippocampus Nerve Tissue Proteins Hippocampal formation Biology Mice Postsynaptic potential Memory Syntaxin Animals Episodic memory Mice Inbred BALB C Multidisciplinary Microfilament Proteins Estrogens Dendrites Biological Sciences Immunohistochemistry Mice Inbred C57BL nervous system Space Perception Immunology Synapses Ovariectomized rat Female Neuroscience Biomarkers |
| Popis: | Estrogen (E) treatment induces axospinous synapses in rat hippocampus in vivo and in cultured hippocampal neurons in vitro . To better explore the molecular mechanisms underlying this phenomenon, we have established a mouse model for E action in the hippocampus by using Golgi impregnation to examine hippocampal dendritic spine morphology, radioimmunocytochemistry (RICC) and silver-enhanced immunocytochemistry to examine expression levels of synaptic protein markers, and hippocampal-dependent object-placement memory as a behavioral readout for the actions of E. In ovariectomized mice of several strains and F 1 hybrids, the total dendritic spine density on neurons in the CA1 region was not enhanced by E treatment, a finding that differs from that in the female rat. E treatment of ovariectomized C57BL/6J mice, however, caused an increase in the number of spines with mushroom shapes. By RICC and silver-enhanced immunocytochemistry, we found that the immunoreactivity of postsynaptic markers (PSD95 and spinophilin) and a presynaptic marker (syntaxin) were enhanced by E treatment throughout all fields of the dorsal hippocampus. In the object-placement tests, E treatment enhanced performance of object placement, a spatial episodic memory task. Taken together, the morphology and RICC results suggest a previously uncharacterized role of E in synaptic structural plasticity that may be interpreted as a facilitation of the spine-maturation process and may be associated with enhancement of hippocampal-dependent memory. |
| Databáze: | OpenAIRE |
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