| Autor: |
Huguet, Florentin, Gokham, Ezgi, Foster, Helen A., Amin, Hasnat A., Vagnarelli, Paola |
| Rok vydání: |
2022 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.19410310 |
| Popis: |
A) Sequence alignment between Repo-Man wt and the IRCE mutant generated. B) GFP pull-down on HeLa cells transfected with either GFP or GFP:RepoMan wt or GFP:RepoMan IRCE. The samples were run on SDS PAGE, blotted and probed with either GFP antibody (left panel) or anti SUMPO2/3 antibody (right panel). UB=unbound fraction B=bound fraction. C) HeLa cells were transfected with either GFP or GFP:RepoManwt. The GFP constructs were pulled-down with GFP-binder beads and incubated with recombinant purified lamin A. Western blot of the unbound (UB) and bound (B) fractions probed with anti GFP (Bottom panel), lamin A (middle panel) and RepoMan (top panel) antibodies. D) Quantification of the mitotic progression (1���3 h from release) of the HCT116 RepoMan:FKBP:GFP (green) / pMito-mCherry-FRB (red) cell line with and without rapamycin from the experiment designed in Figure 3 D. between 75���100 cells were analysed per each condition. The datasets with and without rapamycin at each time point were statistically analysed with a Chi Square test. Ns= not significant. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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