An improved in vitro model for studying the structural and functional properties of the endothelial glycocalyx in arteries, capillaries and veins
| Autor: | Jayachandran N. Kizhakkedathu, Sargun Bajaj, Masoud Daneshi, D. Mark Martinez, Erika M. J. Siren, Edward M. Conway, Karen C. Cheung, Haiming D. Luo, Jordan MacKenzie |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Endothelium Microfluidics Inflammation Glycocalyx Biochemistry Veins 03 medical and health sciences 0302 clinical medicine Genetics medicine Human Umbilical Vein Endothelial Cells Humans Molecular Biology Innate immune system Hemodynamics Arteries In vitro Capillaries 030104 developmental biology medicine.anatomical_structure Cell culture Biophysics Human umbilical vein endothelial cell Stress Mechanical medicine.symptom Shear Strength 030217 neurology & neurosurgery Biotechnology Blood vessel |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental BiologyREFERENCES. 35(6) |
| ISSN: | 1530-6860 |
| Popis: | The endothelial glycocalyx is a dynamic structure integral to blood vessel hemodynamics and capable of tightly regulating a range of biological processes (ie, innate immunity, inflammation, and coagulation) through dynamic changes in its composition of the brush structure. Evaluating the specific roles of the endothelial glycocalyx under a range of pathophysiologic conditions has been a challenge in vitro as it is difficult to generate functional glycocalyces using commonly employed 2D cell culture models. We present a new multi-height microfluidic platform that promotes the growth of functional glycocalyces by eliciting unique shear stress forces over a continuous human umbilical vein endothelial cell monolayer at magnitudes that recapitulate the physical environment in arterial, capillary and venous regions of the vasculature. Following 72 hours of shear stress, unique glycocalyx structures formed within each region that were distinct from that observed in short (3 days) and long-term (21 days) static cell culture. The model demonstrated glycocalyx-specific properties that match the characteristics of the endothelium in arteries, capillaries and veins, with respect to surface protein expression, platelet adhesion, lymphocyte binding and nanoparticle uptake. With artery-to-capillary-to-vein transition on a continuous endothelial monolayer, this in vitro platform is an improved system over static cell culture for more effectively studying the role of the glycocalyx in endothelial biology and disease. |
| Databáze: | OpenAIRE |
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