Genistein, a natural phytoestrogen from soy, relieves neuropathic pain following chronic constriction sciatic nerve injury in mice: anti-inflammatory and antioxidant activity
| Autor: | Anna Elisa Valsecchi, Anna Elisa Trovato, Silvia Franchi, Alberto E. Panerai, Paola Sacerdote, Mariapia Colleoni |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Anti-Inflammatory Agents Genistein Estrogen receptor Phytoestrogens Biochemistry Antioxidants Nitric oxide Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Ganglia Spinal Internal medicine medicine Animals Neurons Afferent Receptor Ligation Inflammation Dose-Response Relationship Drug Plant Extracts Antagonist Nociceptors Peripheral Nervous System Diseases Sciatic nerve injury medicine.disease Mice Inbred C57BL Disease Models Animal Oxidative Stress Treatment Outcome Cytokine Endocrinology Receptors Estrogen chemistry Hyperalgesia Chronic Disease Neuropathic pain Soybeans Inflammation Mediators Sciatic Neuropathy |
| Zdroj: | Journal of Neurochemistry. 107:230-240 |
| ISSN: | 1471-4159 0022-3042 |
| Popis: | There is great interest in soy isoflavones as alternatives to endogenous estrogens not only in hormonal pathologies, but also in inflammatory, neurodegenerative diseases, and pain. We investigated the effect of the isoflavone genistein on neuropathic pain. Genistein binds estrogen receptors (ER) with higher affinity for the ERbeta particularly expressed in neuronal and immune cells. Neuropathy was induced in mice by means of chronic sciatic nerve constriction, and the subcutaneous administration of genistein from the third day after the lesion reversed pain hypersensitivity in a time- and dose-dependent manner. This effect may have been due to the activation of classical nuclear receptor and/or anti-oxidant, anti-inflammatory, and immunomodulating properties of genistein. The fact that a specific ERbeta antagonist prevented both its anti-allodynic and anti-hyperalgesic action, whereas a specific ERalpha antagonist was ineffective and a non-selective ER antagonist only reversed the anti-allodynic effect, suggests the involvement of ERbeta. Antioxidant effects are also involved as the anti-nociceptive dose reversed the increase in reactive oxygen species and malondialdehyde in injured paw tissues, and increased the activity of anti-oxidant enzymes. The phytoestrogen had immunomodulatory and anti-inflammatory activities as it reduced peripheral and central nuclear factor-kappaB, nitric oxide system and pro-inflammatory cytokine over-activation. Taken together, our results suggest that genistein could ameliorate painful neuropathy by multiple mechanisms. |
| Databáze: | OpenAIRE |
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