| Autor: |
Hsuan Cheng, Yu‐Lin Kao, Ting Chen, Lohitaksh Sharma, Wen‐Ting Yang, Yi‐Chien Chuang, Shih‐Han Huang, Hong‐Rui Lin, Yao‐Shen Huang, Chi‐Ling Kao, Lee‐Wei Yang, Rachel Bearon, Hui‐Chun Cheng, Kuo‐Chiang Hsia, Yu‐Chun Lin |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
EMBO reportsReferences. |
| ISSN: |
1469-3178 |
| Popis: |
The centrosome, a non-membranous organelle, constrains various soluble molecules locally to execute its functions. As the centrosome is surrounded by various dense components, we hypothesized that it may be bordered by a putative diffusion barrier. After quantitatively measuring the trapping kinetics of soluble proteins of varying size at centrosomes by a chemically inducible diffusion trapping assay, we find that centrosomes are highly accessible to soluble molecules with a Stokes radius of less than 5.8 nm, whereas larger molecules rarely reach centrosomes, indicating the existence of a size-dependent diffusion barrier at centrosomes. The permeability of this barrier is tightly regulated by branched actin filaments outside of centrosomes and it decreases during anaphase when branched actin temporally increases. The actin-based diffusion barrier gates microtubule nucleation by interfering with γ-tubulin ring complex recruitment. We propose that actin filaments spatiotemporally constrain protein complexes at centrosomes in a size-dependent manner. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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