Incorporation of high molecular weight RNA into large artificial lipid vesicles
| Autor: | Dario Giacomoni, Marc J. Ostro, Sheldon Dray |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
food.ingredient
Size-exclusion chromatography Biophysics Ether Biochemistry Lecithin Permeability Sepharose chemistry.chemical_compound food Ribonucleases Escherichia coli Ribonuclease Molecular Biology Liposome Chromatography biology Cholesterol RNA Cell Biology Molecular Weight Microscopy Electron RNA Bacterial chemistry Liposomes biology.protein |
| Zdroj: | Biochemical and biophysical research communications. 76(3) |
| ISSN: | 0006-291X |
| Popis: | By utilizing an ether infusion technique with lecithin, cholesterol, and dicetylphosphate, giant liposomes have been produced with diameters ranging from 0.5 to 2.0 microns. These liposomes have been used to sequester 4S, 16S, and 23S E. coli [3H]RNA and can be effectively separated from non-liposome incorporated RNA by ribonuclease treatment followed by Sepharose 4B gel filtration. The [3H]RNA within these liposomes can be extracted and appears to be undegraded. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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