Preclinical evaluation of an innovative bone graft of marine origin for the treatment of critical-sized bone defects in an animal model
| Autor: | Julia Serra, Mónica López-Peña, Fernando Muñoz, María Permuy, Miriam López-Álvarez, Pío González, Estefanía López-Senra, Antonio González-Cantalapiedra, Rafael Otero-Pérez |
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| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Anatomía, Produción Animal e Ciencias Clínicas Veterinarias |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Biocompatibility
Bone tissue lcsh:Technology Osseointegration calcium phosphate lcsh:Chemistry 03 medical and health sciences 0302 clinical medicine Animal model Marine bone graft Fibrosis Preclinical trial Medicine General Materials Science Bone regeneration Instrumentation lcsh:QH301-705.5 Fluid Flow and Transfer Processes Bone mineral 030222 orthopedics 2407.01 Cultivo Celular business.industry lcsh:T Process Chemistry and Technology General Engineering biomaterial Biomaterial osseointegration 3314.02 Prótesis 030206 dentistry medicine.disease preclinical trial lcsh:QC1-999 3312 Tecnología de Materiales Computer Science Applications medicine.anatomical_structure Calcium phosphate lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 marine bone graft business lcsh:Engineering (General). Civil engineering (General) lcsh:Physics Biomedical engineering |
| Zdroj: | Applied Sciences Volume 11 Issue 5 Investigo. Repositorio Institucional de la Universidade de Vigo Universidade de Vigo (UVigo) Applied Sciences, Vol 11, Iss 2116, p 2116 (2021) Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname Minerva: Repositorio Institucional de la Universidad de Santiago de Compostela Universidad de Santiago de Compostela (USC) |
| Popis: | Autogenous cancellous bone graft is the current gold standard of treatment for the management of bone defects since it possesses the properties of osteoinduction, osteoconduction, and osteogenesis. Xenografts and synthetic grafts have been widely reported as available and low-cost alternatives, which retain good osteoconductive and mechanical properties. Given the rich biodiversity of ocean organisms, marine sources are of particular interest in the search for alternative bone grafts with enhanced functionalities. The purpose of this paper is to assess the biocompatibility of a marine-derived bone graft obtained from shark tooth, which is an environmentally sustainable and abundant raw material from fishing. This research presents the findings of a preclinical trial—following UNE-EN ISO 10993—that induced a critical-sized bone defect in a rabbit model and compared the results with a commercial bovine-derived bone graft. Evaluation by micro-computed tomography and histomorphometric analysis 12 weeks after implantation revealed good osseointegration, with no signs of inflammatory foreign body reactions, fibrosis, or necrosis in any of the cases. The shark tooth-derived bone graft yielded significantly higher new bone mineral density values (54 ± 6%) than the control (27 ± 8%). Moreover, the percentage of intersection values were much higher (86 ± 8%) than the bovine-derived bone graft (30 ± 1%) used as control. The area of occupancy by bone tissue in the test material (38 ± 5%) also gave higher values than the control (30 ± 6%). The role of physicochemical properties, biphasic structure, and composition on the stimulation of bone regeneration is also discussed This research was funded by Xunta de Galicia, grants IN855A 2016/06 (Programa Ignicia–GAIN), ED431C 2017/51 (Competitive Reference Groups) and ED431D 2017/13 (Research networks); and by European Union Interreg Programs, projects IBEROS (0245_IBEROS_1_E, POCTEP 2015), CVMar+i (0302_CVMAR_I_1_P, POCTEP 2015) and BLUEHUMAN (EAPA_151/2016, Atlantic Area 2016) SI |
| Databáze: | OpenAIRE |
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