Expression of mucosa-related integrin αEβ7 on alveolar T cells in interstitial lung diseases
| Autor: | Werner Seeger, R Tenter, H. G. Velcovsky, Jürgen Lohmeyer, H. Morr, J. Friedrich, U. Maus, F. Grimminger, Simone Rosseau |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Integrins Pathology medicine.medical_specialty Adolescent Sarcoidosis Pulmonary Fibrosis T cell Immunology CD8-Positive T-Lymphocytes Idiopathic pulmonary fibrosis Transforming Growth Factor beta medicine Humans Immunology and Allergy Aged Lung medicine.diagnostic_test business.industry Respiratory disease Interstitial lung disease Original Articles Middle Aged respiratory system medicine.disease respiratory tract diseases Bronchoalveolar lavage medicine.anatomical_structure Female business Bronchoalveolar Lavage Fluid Hypersensitivity pneumonitis CD8 Alveolitis Extrinsic Allergic |
| Zdroj: | Clinical and Experimental Immunology. 116:340-346 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.1999.00894.x |
| Popis: | The expression of alphaEbeta7 integrin has been related to the selective retention of lymphocytes in mucosal tissues of gut, urogenital tract and lung. To identify potential disease-associated alphaEbeta7 expression patterns on cells accounting for lymphocytic alveolitis in interstitial lung disease (ILD), alphaE expression on CD4+ and CD8+ T cell subsets was evaluated by dual-colour flow cytometry in peripheral blood and bronchoalveolar lavage fluid (BALF) of patients with idiopathic pulmonary fibrosis (IPF; n = 18), hypersensitivity pneumonitis (HP; n = 20) and sarcoidosis (n = 44) in comparison with healthy controls (n = 15). In both healthy individuals and all patient groups the proportion of alphaE-bearing T cells in peripheral blood was < 2%, whereas the vast majority of alveolar CD8+ T cells consistently co-expressed alphaE. Absolute alveolar CD8+alphaE+ cell numbers/ml were up to 30-fold increased in HP patients. Proportions of alphaE-bearing CD4+ cells in BALF were significantly elevated in IPF (74.0 +/- 2.7%) and HP (70.0 +/- 2.4%) compared with normals (30.0 +/- 1.8%) (mean +/- s.e.m.; P < 0.01). In sarcoidosis, the alphaE expression on BALF CD4+ cells displayed subgroup dependency: proportions significantly lower than normal were noted in chest radiographic stage I (14.3 +/- 1.5%), but increased proportions in stages II (50.0 +/- 3.8%) and III (64.0 +/- 4.8%). Correlations between common markers of T cell activation or BALF transforming growth factor-beta (TGF-beta ) bioactivity and alphaE expression were not noted. We conclude that the vast majority of alveolar CD8+ T cells consistently express alphaEbeta7 and that distinct patterns of alphaEbeta7 expression on alveolar CD4+ lymphocytes in sarcoidosis are related to the diverse manifestations of the sarcoid inflammatory process in the lung. |
| Databáze: | OpenAIRE |
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