Fluorescence fibre-optic confocal microscopy of skin in vivo: microscope and fluorophores
| Autor: | Jørgen Serup, Steven G. Thomas, Christian Suihko, Lucinda D. Swindle |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Fluorophore
Microscope Materials science Technology Assessment Biomedical Confocal Human skin Dermoscopy Dermatology Sensitivity and Specificity law.invention chemistry.chemical_compound Optics Dermis Confocal microscopy law Image Interpretation Computer-Assisted medicine Fiber Optic Technology Fluorescein Fluorescent Dyes Microscopy Confocal integumentary system business.industry Reproducibility of Results Signal Processing Computer-Assisted Equipment Design Image Enhancement Fluorescence Equipment Failure Analysis medicine.anatomical_structure chemistry Microscopy Fluorescence business Biomedical engineering |
| Zdroj: | Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI). 11(4) |
| ISSN: | 0909-752X |
| Popis: | Background/aims: Fibre-optic confocal imaging in vivo is a new approach in the assessment of human skin. The objective is to describe a novel instrument and its operation and use in combination with fluorophores. Methods: The Stratum® is a fibre-optic fluorescence confocal microscope especially developed for the study of skin and mucous membranes. The system is flexible and any body site can be studied with a hand-held scanner. The light source is a 488 nm argon ion laser. Horizontal (en face) images of the epidermis and outer dermis are produced with cellular resolution. Magnification is approximately 1000x. Fluorescein sodium is routinely used as fluorophore (intradermal injection or application to the skin surface). This fluorophore is safe for human use in vivo, but other substances (rhodamine B, Acridine Orange, green fluorescent protein, curcumin) have also been studied. Results: The instrument produces sharp images of epidermal cell layers from the epidermal surface to the sub-papillary dermis, with sub-cellular resolution. The scanner is flexible in use. The technique of intradermal fluorophore injection requires some skill. Conclusions: We consider this fibre-optic instrument a potentially important tool in skin research for non-invasive optical biopsy of primarily the epidermis. Present use is focussed on research applications, where the fluorophore distribution in the skin may illustrate morphological changes in the epidermis. © Blackwell Munksgaard, 2005. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|