Development of a radioimmunoassay for human platelet factor 4
| Autor: | Shirley P. Levine, Linda S. Krentz |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Immunodiffusion
Radioimmunoassay Centrifugation Immunoelectrophoresis Platelet Factor 4 Antibodies Affinity chromatography In vivo medicine Animals Humans Platelet-poor plasma Blood Specimen Collection Chromatography medicine.diagnostic_test Heparin Chemistry Anticoagulants Hematology Blood Coagulation Factors Electrophoresis Polyacrylamide Gel Platelet lysate Rabbits Platelet factor 4 Protein Binding |
| Zdroj: | Thrombosis Research. 11:673-686 |
| ISSN: | 0049-3848 |
| DOI: | 10.1016/0049-3848(77)90025-1 |
| Popis: | Human platelet factor 4 (PF4), a secreted protein with antiheparin activity, was purified by affinity chromatography on heparinepsilon aminocaproic acid-SepharoseR and used to prepare antibodies in rabbits. The resulting antibodies reacted in immunodiffusion with purified PF4, platelet lysate, and serum and were monospecific by immunoelectrophoresis. Purified PF4 was radioiodinated with 125I by the chloramine-T method and used to develop a radioimmunoassay that was rapid, accurate, and sensitive enough to measure 0.73 ng of PF4. Normal values in platelet poor plasma collected into ethylene-diamine-tetraacetate (EDTA), dibutyryl cyclic adenosine 3′5′ monophosphoric acid (db-cAMP), and prostaglandin E1 (PGE1) were 4.7 ± 2.5 ng/ml (mean ± S.D.). Levels in serum from clotted whole blood were 13,200 ± 3,200 ng/ml. This assay should serve as a sensitive indicator of both in vivo and in vitro platelet release. |
| Databáze: | OpenAIRE |
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