Characterization of the Expression of TLR2 (Toll-like Receptor 2) and TLR4 on Circulating Monocytes in Coronary Artery Disease
| Autor: | Tadayuki Yakushiji, Kazuhiro Ashida, Hironori Tsujimoto, Astushi Yonemura, Hoshio Hiraide, Masatoshi Kusuhara, Noriyuki Iwamoto, Koji Miyazaki, Eiji Takayama, Hidetaka Mochizuki, Kikuo Isoda, Fumitaka Ohsuzu, Makoto Ayaori |
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| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Lipopolysaccharide Receptors Cell Surface Peripheral blood mononuclear cell Fluorescence Monocytes Angina Pectoris chemistry.chemical_compound Internal medicine Internal Medicine medicine Humans Receptor Aged Aged 80 and over Toll-like receptor Membrane Glycoproteins Unstable angina business.industry Monocyte Toll-Like Receptors Biochemistry (medical) Middle Aged Flow Cytometry medicine.disease Toll-Like Receptor 2 Toll-Like Receptor 4 TLR2 medicine.anatomical_structure Endocrinology chemistry Immunology TLR4 Female Cardiology and Cardiovascular Medicine business |
| Zdroj: | Journal of Atherosclerosis and Thrombosis. 12:53-60 |
| ISSN: | 1880-3873 1340-3478 |
| DOI: | 10.5551/jat.12.53 |
| Popis: | TLRs are receptors involved in the recognition of pathogens by the innate immune system, and TLR2 and TLR4 play important roles in the activation of monocytes. A total of 105 consecutive patients who underwent coronary angiography comprised of 46 with stable effort angina (SA), 41 with unstable angina (UA), and 18 with no significant CAD (CNT) were enrolled. The baseline expression levels of TLR2 and TLR4 on monocytes in peripheral blood mononuclear cells (PBMCs) were determined by flow-cytometric analysis. Since TLR2 expression has been reported to be regulated by TLR4 signaling, we cultured PBMCs with or without lipopolysaccharide (LPS, 1 microg/ml). At baseline, TLR4 levels (mean of fluorescence intensity ) in SA (145 +/- 58, p < 0.05) and UA (164 +/- 65, p < 0.01) were higher than those in CNT (107 +/- 37). As for TLR2, levels were higher in UA (108 +/- 36, p < 0.05) than in SA (94 +/- 18) and CNT (87 +/- 22). After stimulation with LPS, TLR2 levels increased in SA but decreased in UA. In conclusions, TLR4 levels increased in both SA and UA. Monocytes in UA were characterized by elevated TLR2 levels and unresponsiveness of the TLR2 levels to TLR4 stimulation. |
| Databáze: | OpenAIRE |
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