Decreased Levels of Circulating CD4+CD25+Foxp3+ Regulatory T Cells in Patients with Primary Antiphospholipid Syndrome
| Autor: | Ester Rosári Raphaelli Dal Ben, Carine Hartmann do Prado, Henrique Luiz Staub, Moisés Evandro Bauer, Talita Siara Almeida Baptista |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.drug_class CD3 Antigens CD19 Immunology Cell chemical and pharmacologic phenomena Lymphocyte Activation medicine.disease_cause Monoclonal antibody T-Lymphocytes Regulatory Immunophenotyping Autoimmunity Flow cytometry T-Lymphocyte Subsets Antiphospholipid syndrome Internal medicine Humans Immunology and Allergy Medicine Lymphocyte Count IL-2 receptor B-Lymphocytes medicine.diagnostic_test biology business.industry Interleukin-2 Receptor alpha Subunit FOXP3 Forkhead Transcription Factors hemic and immune systems Middle Aged Antiphospholipid Syndrome medicine.disease Cross-Sectional Studies Endocrinology medicine.anatomical_structure Blood Circulation CD4 Antigens biology.protein Female business |
| Zdroj: | Journal of Clinical Immunology. 33:876-879 |
| ISSN: | 1573-2592 0271-9142 |
| DOI: | 10.1007/s10875-012-9857-y |
| Popis: | CD4(+)CD25(+)Foxp3(+) regulatory T (Treg) cell dysfunction has been documented in various autoimmune disorders, but not in antiphospholipid syndrome (APS) so far.In this cross-sectional study, we aim to investigate CD4(+)CD25(+)Foxp3(+) Treg cells, CD3(+)CD19(-) T cells and CD3(-)CD19(+) B cells in patients with primary APS and healthy controls. Cell subtypes were immunophenotyped using specific monoclonal antibodies (anti-CD3 CY5, anti-CD4 FITC, anti-CD25, anti-Foxp3, anti-CD19 PE) and flow cytometry.Twenty patients with APS and 20 age- and sex-matched controls were studied. The percentage of total lymphocytes, activated Th cells (CD4+CD25+), Treg cells and CD3(-)CD19(+) B cells were found significantly lower in APS patients as compared to controls (all p 0.05).A dysfunction in CD4(+)CD25(+)Foxp3(+) Treg cells may represent one of the mechanisms leading to autoimmunity in APS patients. The decreased number of CD3(-)CD19(+) B cells of APS patients warrants further elucidation. |
| Databáze: | OpenAIRE |
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