The Effect of Reduced Neuromuscular Activity on Class IIa Histone Deacetylase and Selected Downstream Pathways
| Autor: | Reza Gharakhanlou, Amir Bahador Dakhili, Mahdieh Molanouri Shamsi, Hamid Rajabi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
lcsh:R5-920
medicine.medical_specialty Chemistry Muscular atrophy Hindlimb Hindlimb Suspension medicine.disease_cause medicine.disease Hindlimb suspension HDAC4 Weight-bearing Atrophy Endocrinology Histone Deacetylase Internal medicine Gene expression medicine Histone deacetylase lcsh:Medicine (General) Myogenin |
| Zdroj: | International Journal of Basic Science in Medicine, Vol 3, Iss 1, Pp 25-31 (2018) |
| ISSN: | 2476-664X |
| DOI: | 10.15171/ijbsm.2018.05 |
| Popis: | Background: Decreased neuromuscular activity, in terms of different models including hindlimb suspension, can result in muscular atrophy by changing different genes expression. Hdac4&5 and their downstream pathways are the two important factors involved in the preservation of muscle mass. Methods: In the current study, in order to survey the changes of Hdac4&5 and their downstream cascade mRNA expression, Wistar rats were assigned into two groups 1) weight bearing (WB) and 2) Hindlimb-suspension (HS) (n=5 in each group). Hindlimb’s rats were suspended for 14 days. After two weeks, the rats were sacrificed and the soleus and plantaris muscles were collected. Thereafter, gene expression was measured using Real time technique. Results: The results showed that 14 days hindlimb suspension decreased muscle mass in both the plantaris and soleus muscles and the latter changes definitely outweighed the former. In addition, it has been shown that the cross-sectional area of the muscle fibers of the plantaris and soleus muscles decreased and, with changes in muscle mass, the observed decrease in soleus was higher. Also, the increase in mRNA expression of Hdac4&5, Myogenin, and Gadd45a, was observed in both muscles. But the expression of the Dach2 gene was significantly reduced in the plantaris and soleus muscles of the HS group as compared to the WB group. Discussion: Hdac/Myogenin and Hdac/Dach2/Gadd45a are the two involved pathways in muscular atrophy process as a result of unloading and this could be considered as treatment candidates to resist muscular atrophy in some specific conditions such as bed rest and space flight. |
| Databáze: | OpenAIRE |
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