Bioautographic screening of Xanthine Oxidase inhibition and antioxidant activities for bioprocess control of in vitro cultivated medicinal and aromatic plants of the Balkan region
| Autor: | Evelyn Wolfram, Beat Meier, Ina Aneva, Simona Pedrussio, Ljuba Evstatieva, Sarah Bräm, Kalina Danova, Rita Könye |
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| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Pulsatilla montana medicine.medical_treatment Aromatic plants Pharmaceutical Science Biology Analytical Chemistry Artemisia alba 615: Pharmakologie und Therapeutik chemistry.chemical_compound In vitro cultivation Drug Discovery Botany medicine Bioprocess Xanthine oxidase Sideritis scardica Pharmacology Organic Chemistry biology.organism_classification In vitro Hypericum calycinum HPTLC bioautography Complementary and alternative medicine chemistry Molecular Medicine |
| Zdroj: | Planta medica |
| ISSN: | 1439-0221 0032-0943 |
| Popis: | In vitro cultivation is considered as a prospective supplementary agriculture and season independent approach for supply of plant raw material [1]. We report here on intermediate results of research activities that aim at development of an in vitro collection of medicinal and aromatic plants from the Balkan region. Optimizations of medium composition as well as development of callus suspension and root cultures are being conducted in order to stimulate production of secondary metabolites with phytopharmaceutical value. Bioprocess control strategies are needed for rapid and cost effective screening for selection of highly productive in vitro lines for later scale up in bioreactor conditions. HPTLC fingerprinting coupled with DPPH ß carotene/linoleic acid bleaching [2] as well as bioautographic Xanthine Oxidase Inhibition [34] assays were applied in order to assess secondary metabolite and active compound content simultaneously. Different in vitro lines of Balkan endemic Sideritis scardica Griseb. Pulsatilla montana ssp. balcana essential oil bearing Artemisia alba Turra as well as several representatives of the Hypericum genus differing in their biosynthetic capacity for hypericines have been studied. Hypericine non producing Hypericum calycinum L. showed superior antioxidant activity as compared with the other species. In vitro cultured S. scardica originating from wild accessions showed higher activity than from field cultivation. From all tested species A. alba showed several inhibitory zones in the HPTLC coupled Xanthine Oxidase Inhibition screening. Further analysis of these zones is feasible by coupled HPTLC MS. The applied bioautographic bioprocess control methods provide for rapid and efficient targeting of prospective highly producing in vitro lines for future up scaling especially when a large number of culture variants have to be handled. Acknowledgements: SNF No. IZEBZ0_142989 and SD MEYS No. DO2 – 1153 |
| Databáze: | OpenAIRE |
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