n' Protein activator sites of plasmid pBR322 are not essential for its DNA replication
| Autor: | Peter Weisbeek, A van der Ende, S. Brenner, Renske Teertstra |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
DNA Replication
DNA Bacterial DNA polymerase II DNA replication Gene Amplification DNA Single-Stranded DNA Restriction Enzymes Biology Primosome Molecular biology DNA polymerase delta Plasmid SeqA protein domain Bacterial Proteins Structural Biology Transduction Genetic Operon biology.protein Escherichia coli DNA supercoil Chromosome Deletion Molecular Biology Replication protein A Plasmids Transcription Factors |
| Zdroj: | Journal of molecular biology, 167(3), 751-756. Academic Press Inc. |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/s0022-2836(83)80108-9 |
| Popis: | The lagging strand DNA synthesis of the Escherichia coli bacterial chromosome and plasmids is thought to be initiated by the mobile promotor, the primosome. This primosome is assembled at a specific site on single-stranded DNA. This process is initiated by the interaction of one of the at least seven components, the n′ protein, with this site. Indeed n′ protein activator sites are found in the plasmids Col E1 and pBR322. To investigate the in vivo function of these n′ protein sites, deletion derivates of pBR322 were constructed in which the n′ protein sites are removed. The deletion plasmids show no change in stability and only threefold reduction in copy number compared to pBR322. Using a transduction system for single-stranded plasmid DNA it was shown that no other specific initiation signals for lagging strand DNA synthesis were present in the deletion plasmids. It was concluded that the n′ protein activator sites in pBR322 are not essential for its DNA replication in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|