La Crosse virus soluble cell culture antigen
| Autor: | J F Obijeski, H S Lindsey, R A Klimas |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Microbiology (medical)
Counterimmunoelectrophoresis Immunodiffusion La Crosse virus viruses Complement Fixation Tests Heterologous Encephalitis Virus California Cross Reactions Biology Complement fixation test Virology Molecular biology Encephalitis Viruses Capsid Antigen Antigens Viral Polyacrylamide gel electrophoresis Ammonium sulfate precipitation Research Article |
| Zdroj: | Journal of Clinical Microbiology. 6:618-626 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.6.6.618-626.1977 |
| Popis: | A virus-free soluble antigen, obtained by ammonium sulfate precipitation of the supernatant fluids of La Crosse virus-infected BHK-21 cell cultures, was more reactive and more specific than infected suckling mouse brain antigen when compared by immunodiffusion and counterelectrophoresis tests. By complement fixation tests, the antigen was cross-reactive with heterologous California group arbovirus hyperimmune mouse ascitic fluids, but to a lesser degree than was the standard sucrose-acetone-extracted infected suckling mouse brain antigen. The major virion nucleocapsid protein of La Crosse virus was found by polyacrylamide gel electrophoresis to be the soluble antigen protein responsible for precipitation in immunodiffusion and counterelectrophoresis tests. |
| Databáze: | OpenAIRE |
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