Elastic Properties of the Cell Surface and Trafficking of Single AMPA Receptors in Living Hippocampal Neurons
Autor: | Frank Lafont, Stefan Catsicas, Sandor Kasas, Alexandre Yersin, Karina Kulangara, Charles Roduit, Giovanni Dietler, Pascal Steiner, Harald Hirling |
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Rok vydání: | 2007 |
Předmět: |
Membrane Fluidity
media_common.quotation_subject Biophysics Stimulation AMPA receptor Hippocampal formation Microscopy Atomic Force Hippocampus Cell membrane 03 medical and health sciences 0302 clinical medicine medicine Receptors AMPA Internalization Receptor 030304 developmental biology media_common Neurons 0303 health sciences Chemistry Cell Membrane Glutamate receptor Elasticity Protein Transport medicine.anatomical_structure Biochemistry nervous system Cell Biophysics NMDA receptor Stress Mechanical 030217 neurology & neurosurgery |
Zdroj: | Biophysical Journal. 92(12):4482-4489 |
ISSN: | 0006-3495 |
DOI: | 10.1529/biophysj.106.092742 |
Popis: | Although various approaches are routinely used to study receptor trafficking, a technology that allows for visualizing trafficking of single receptors at the surface of living cells remains lacking. Here we used atomic force microscope to simultaneously probe the topography of living cells, record the elastic properties of their surface, and examine the distribution of transfected α-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA)-type glutamate receptors (AMPAR). On nonstimulated neurons, AMPARs were located in stiff nanodomains with high elasticity modulus relative to the remaining cell surface. Receptor stimulation with N-methyl-d-aspartate (NMDA) provoked a permanent disappearance of these stiff nanodomains followed by a decrease (53%) of the number of surface AMPARs. Blocking electrical activity before NMDA stimulation recruited the same number of AMPARs for internalization, preceded by the loss of the stiff nanodomains. However, in that case, the stiff nanodomains were recovered and AMPARs were reinserted into the membrane shortly after. Our results show that modulation of receptor distribution is accompanied by changes in the local elastic properties of cell membrane. We postulate, therefore, that the mechanical environment of a receptor might be critical to determine its specific distribution behavior in response to different stimuli. |
Databáze: | OpenAIRE |
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