Influence of dietary eicosapentaenoic acid on the cardiac mechanical and mitochondrial functions during early sepsis
| Autor: | Luc Demaison, Thibault Leger, Jean-Paul Rigaudière, Kasra Azarnoush |
|---|---|
| Přispěvatelé: | Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), service de chirurgie cardiaque, CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Centre Hospitalier Universitaire Gabriel Montpied |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
medicine.medical_specialty
Oxidative phosphorylation heart Mitochondrion Sepsis sepsis icosapentaenoic acid In vivo Internal medicine Medicine 2. Zero hunger chemistry.chemical_classification acide eicosapentaénoique business.industry fonction mitochondriale medicine.disease Eicosapentaenoic acid Endocrinology cœur chemistry Heart failure lipids (amino acids peptides and proteins) acide gras polyinsaturé n-3 Cardiology and Cardiovascular Medicine business Ligation [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Polyunsaturated fatty acid |
| Zdroj: | Printemps de la Cardiologie Recherche Fondamentale & Clinique Printemps de la Cardiologie Recherche Fondamentale & Clinique, Apr 2018, Montpellier, France. Elsevier, Archives of Cardiovascular Diseases. Supplements, 10 (2), pp.178-179, 2018, Archives of Cardiovascular Diseases. Supplements. ⟨10.1016/j.acvdsp.2018.02.008⟩ Printemps de la Cardiologie Recherche Fondamentale & Clinique, Apr 2018, Montpellier, France. Elsevier, 10 (2), pp.178-179, 2018, Archives of Cardiovascular Diseases. Supplements. ⟨10.1016/j.acvdsp.2018.02.008⟩ |
| DOI: | 10.1016/j.acvdsp.2018.02.008⟩ |
| Popis: | Topic 1 — Inflammation — A Topic 1 — Inflammation — ATopic 1 — Inflammation — A; IntroductionSepsis triggers the failure of cardiac mitochondria and this deleterious effect could be prevented by omega-3 polyunsaturated fatty acids (PUFA).ObjectivesTo evaluate the influence of eicosapentaenoic acid (C20: 5 omega-3 or EPA) on the mechanical and mitochondrial functions of the heart during early sepsis.MethodsFour month-old female Wistar rats were subdivided in 4 groups (n = 8 per group) according to: (i) the 4-week diet given (deficient in omega-3 PUFA [DEF] or enriched with EPA); (ii) the surgery performed (caecal ligation at 1 cm of the apex of the heart and puncture in order to induce sepsis [CLP] or sham). The cardiac mechanical function was studied in vivo by NMR 48 h after the surgery. Immediately after, part of the myocardium was used in order to extract mitochondria and another part was frozen in order to subsequently analyse the fatty acid composition of cardiac phospholipids.ResultsThe EPA groups display a large decrease in the omega-6/omega-3 PUFA ratio of cardiac phospholipids. Sepsis increases the mRNA expression of IL-1beta in the DEF group but not in the EPA one. The mechanical function is altered neither by the diets nor by the surgeries. The DEF/CLP animals have a deficient oxidative phosphorylation compared to the DEF/Sham rats: reduced mitochondrial oxygen consumption (−42%, P < 0.001) and ATP production (−37%, P < 0.05). The sepsis-induced mitochondrial damages in this group is due to an increase reactive oxygen species (ROS) release (+130 and +63% for the ROS/respiration and ROS/ATP ratios, P < 0.01 and P < 0.05, respectively). The dietary EPA prevents these damages (P < 0.05).ConclusionA sufficient EPA intake favours the upholding of the mitochondrial function during early sepsis, which could delay the subsequent development of heart failure. We plan to verify this beneficial effect of EPA in Humans affected by acute endocarditis. |
| Databáze: | OpenAIRE |
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