Lymph node formation and B cell homeostasis require IKK-α in distinct endothelial cell-derived compartments
| Autor: | Michelle D Cully, Yinling Hu, Michael P. Cancro, Nipun Jayachandran, Ellen Puré, Michael J. May, Bruce D. Freedman, Jorge I. Alvarez, James Monslow, Jacquelyn Freund-Brown, Kelly A. McCorkell, Tiffany Weinkopff |
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| Rok vydání: | 2021 |
| Předmět: |
Male
Stromal cell Lymphoid Tissue cells Organogenesis Protein Serine-Threonine Kinases environment and public health Cell Line Mice NF-KappaB Inhibitor alpha B cell homeostasis Marginal zone B-cell medicine Animals Homeostasis skin and connective tissue diseases Lymph node B cell B-Lymphocytes Multidisciplinary Chemistry Tumor Necrosis Factor-alpha NF-kappa B Endothelial Cells Biological Sciences Cell biology I-kappa B Kinase Endothelial stem cell Mice Inbred C57BL Haematopoiesis enzymes and coenzymes (carbohydrates) Lymphatic system medicine.anatomical_structure Female I-kappa B Proteins Lymph Nodes biological phenomena cell phenomena and immunity Signal Transduction |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Global inactivation of IκB kinase (IKK)-α results in defective lymph node (LN) formation and B cell maturation, and loss of IKK-α–dependent noncanonical NF-κB signaling in stromal organizer and hematopoietic cells is thought to underlie these distinct defects. We previously demonstrated that this pathway is also activated in vascular endothelial cells (ECs). To determine the physiologic function of EC-intrinsic IKK-α, we crossed Ikkα(F/F) mice with Tie2-cre or Cdh5-cre mice to ablate IKK-α in ECs. Notably, the compound defects of global IKK-α inactivation were recapitulated in Ikkα(Tie2) and Ikkα(Cdh5) mice, as both lacked all LNs and mature follicular and marginal zone B cell numbers were markedly reduced. However, as Tie2-cre and Cdh5-cre are expressed in all ECs, including blood forming hemogenic ECs, IKK-α was also absent in hematopoietic cells (HC). To determine if loss of HC-intrinsic IKK-α affected LN development, we generated Ikkα(Vav) mice lacking IKK-α in only the hematopoietic compartment. While mature B cell numbers were significantly reduced in Ikkα(Vav) mice, LN formation was intact. As lymphatic vessels also arise during development from blood ECs, we generated Ikkα(Lyve1) mice lacking IKK-α in lymphatic ECs (LECs) to determine if IKK-α in lymphatic vessels impacts LN development. Strikingly, while mature B cell numbers were normal, LNs were completely absent in Ikkα(Lyve1) mice. Thus, our findings reveal that IKK-α in distinct EC-derived compartments is uniquely required to promote B cell homeostasis and LN development, and we establish that LEC-intrinsic IKK-α is absolutely essential for LN formation. |
| Databáze: | OpenAIRE |
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