Inactivated Nevus Tissue with High Hydrostatic Pressure Treatment Used as a Dermal Substitute after a 28-Day Cryopreservation Period
| Autor: | Naoki Morimoto, Michiharu Sakamoto, Jun Arata, Yoshitaka Matsuura, Shuichi Ogino |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Pathology medicine.medical_specialty Skin Neoplasms Article Subject Hydrostatic pressure Mice Nude General Biochemistry Genetics and Molecular Biology Cryopreservation Extracellular matrix Mice 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Dermis Fresh Tissue Hydrostatic Pressure Animals Humans Medicine Nevus skin and connective tissue diseases Skin Artificial General Immunology and Microbiology business.industry 030208 emergency & critical care medicine General Medicine medicine.disease medicine.anatomical_structure Cryopreserved Tissue Epidermis business Research Article |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2021 (2021) |
| ISSN: | 2314-6133 |
| DOI: | 10.1155/2021/3485189 |
| Popis: | Background. Giant congenital melanocytic nevi (GCMN) treatment remains controversial. While surgical resection is the best option for complete removal, skin shortage to reconstruct the skin defect remains an issue. We report a novel treatment using a high hydrostatic pressurization (HHP) technique and a cryopreservation procedure. However, cryopreservation may inhibit revascularization of implanted nevus tissue and cultured epidermal autograft (CEA) take. We aimed to investigate the influence of the cryopreservation procedure on the HHP-treated dermis specimen and CEA take on cryopreserved tissue. Methods. Nevus tissue harvested from a patient with GCMN was inactivated with HHP of 200 MPa and then cryopreserved at -30°C for 28 days. The cryopreserved specimen was compared with fresh (HHP-treated without cryopreservation) tissue and with untreated (without HHP treatment) tissue to evaluate the extracellular matrix, basal membranes, and capillaries. Cultured epidermis (CE) take on the cryopreserved tissue was evaluated following implantation of the cryopreserved nevus tissue with CE into the subcutis of nude mice. Results. No difference was observed between cryopreserved and fresh tissue in terms of collagen or elastic fibers, dermal capillaries, or basement membranes at the epidermal-dermal junction. In 4 of 6 samples (67%), applied CE took on the nevus tissues and regenerated the epidermis in the cryopreserved group compared with 5 of 6 samples (83%) in the fresh group. Conclusion. Cryopreservation at -30°C for 28 days did not result in significant damage to inactivated nevus tissue, and applied CE on the cryopreserved nevus tissues took and regenerated the epidermis. Inactivated nevus tissue with HHP can be used as a dermal substitute after 28-day cryopreservation. |
| Databáze: | OpenAIRE |
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