A novel signal-on photoelectrochemical immunosensor for detection of alpha-fetoprotein by in situ releasing electron donor
| Autor: | Guang-Chao Zhao, Jiexia Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Streptavidin
Analyte Biomedical Engineering Biophysics Analytical chemistry Electrons Biosensing Techniques 02 engineering and technology 01 natural sciences chemistry.chemical_compound Biotin Limit of Detection Neoplasms Quantum Dots Biomarkers Tumor Electrochemistry Humans Immunoassay Detection limit Photocurrent Chromatography 010401 analytical chemistry General Medicine 021001 nanoscience & nanotechnology Ascorbic acid 0104 chemical sciences chemistry Linear range Nanoparticles Gold alpha-Fetoproteins 0210 nano-technology Alpha-fetoprotein Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 98:155-160 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2017.06.047 |
| Popis: | A signal-on photoelectrochemical (PEC) immunosensor was constructed for detecting tumor marker in this work. α-fetoprotein (AFP) was chosen as a model analyte to investigate the prepared procedure and the analytical performance of the exploited sensor. In order to construct the sensor, CdSe QDs were used as photoactive material, biotin conjugated AFP antibody (Bio-anti-AFP) as detecting probe, streptavidin (SA) as signal capturing unit, biotin functionalized apoferritin encapsulated ascorbic acid (Bio-APOAA) as amplification unit, which were assembled onto the electrodes. The sensing strategy was based on in situ enzymatic hydrolysis of Bio-APOAA to release ascorbic acid (AA) as sacrificial electron donor to produce photocurrent. The photocurrent from the immunosensor was monitored as a result of AFP concentrations. The constructed sensing platform displayed high selectivity and good sensitivity for detecting AFP. Under optimal conditions, a wide linear range from 0.001 to 1000 ng/mL and a low detection limit of 0.31 pg/mL were obtained. The developed immunosensor is expected to be used to determine AFP and other tumor markers in human plasma in clinical laboratories either for pre-cancer screening or cancer monitoring. Moreover, this sensing platform further has the potential to use for the detection of trypsin activity and the corresponding inhibitor-screening. |
| Databáze: | OpenAIRE |
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