Mass-producible microporous silicon membranes for specific leukocyte subset isolation, immunophenotyping, and personalized immunomodulatory drug screening in vitro
| Autor: | Nicolas Mesyngier, Robert Nidetz, Yujing Song, Jianping Fu, Meng Ting Chung, Andrew Stephens, Katsuo Kurabayashi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Silicon
Materials science Surface Properties Microfluidics Biomedical Engineering Drug Evaluation Preclinical chemistry.chemical_element Bioengineering 02 engineering and technology 01 natural sciences Biochemistry Article law.invention Immunophenotyping chemistry.chemical_compound Jurkat Cells law medicine Leukocytes Humans Immunologic Factors Particle Size Cells Cultured Chemiluminescence Polydimethylsiloxane medicine.diagnostic_test 010401 analytical chemistry Pipette General Chemistry Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology 0104 chemical sciences Membrane chemistry Immunoassay 0210 nano-technology Porosity Biomedical engineering |
| Zdroj: | Lab Chip |
| ISSN: | 1473-0189 |
| Popis: | Widespread commercial and clinical adaptation of biomedical microfluidic technologyhas been limited in large part due to the lack of mass producibility of polydimethylsiloxane (PDMS) and glass-based devices commonly as reported in the literature. Here, we present a batch-fabricated, robust, and mass-producible immunophenotyping microfluidic device using silicon micromachining processes. Our Si and glass-based microfluidic device, named the silicon microfluidic immunophenotyping assay (SiMIPA), consists of a highly porous (~ 40%) silicon membrane that can selectively separate microparticles below a certain size threshold. The device is capable of isolating and stimulating specific leukocyte populations, and allows for measuring their secretion of cell signaling proteins by means of a no-wash homogeneous chemiluminescence-based immunoassay. The high manufacturing throughput (~ 170 devices per wafer) makes a large quantity of SiMIPA chips readily available for clinically relevant applications, which normally require large dataset acquisitions for statistical accuracy. With 30 SiMIPA chips, we performed in-vitro immunomodulatory drug screening on isolated leukocyte subsets, yielding 5 data points at 6 drug concentrations. Furthermore, the excellent structural integrity of the device allowed for samples and reagents to be loaded using a micropipette, greatly simplifying the experimental protocol. |
| Databáze: | OpenAIRE |
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