Hypoxia inducible factor -1 regulates WSSV-induced glycolytic genes in the white shrimp Litopenaeus vannamei
| Autor: | Jesús A. Rosas-Rodríguez, Ramona Icedo-García, Melissa M. Miranda-Cruz, Jose A. Godoy-Lugo, José G. Soñanez-Organis, Norma Patricia Adan-Bante |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
White spot syndrome Aquatic Science Biology Arthropod Proteins 03 medical and health sciences chemistry.chemical_compound White spot syndrome virus 1 Penaeidae Gene expression Environmental Chemistry Gene silencing Animals Glycolysis Gene Silencing Regulator gene 04 agricultural and veterinary sciences General Medicine biology.organism_classification Hypoxia-Inducible Factor 1 alpha Subunit Cell biology Shrimp 030104 developmental biology chemistry Gene Expression Regulation 040102 fisheries 0401 agriculture forestry and fisheries Pyruvic acid Phosphofructokinase |
| Zdroj: | Fishshellfish immunology. 92 |
| ISSN: | 1095-9947 |
| Popis: | Hypoxia-inducible factor -1 (HIF-1) is a transcriptional factor that regulates the expression of several glycolytic genes. The white spot syndrome virus (WSSV) induces a shift in glycolysis that favors viral replication in white shrimp Litopenaeus vannamei. HIF-1 is related to the pathogenesis of the WSSV infection through the induction of metabolic changes in infected white shrimp. Although the WSSV infection is associated with metabolic changes, the role of HIF-1 on key glycolytic genes during the WSSV infection has not been examined. In this work, we evaluated the effect of HIF-1α silencing on expression and activity of glycolytic enzymes (Hexokinase-HK, phosphofructokinase-PFK and pyruvate kinase-PK) along with the glucose transporter 1 (Glut1), regulatory enzymes (glucose-6-phosphate dehydrogenase-G6PDH and pyruvate dehydrogenase-PDH), and metabolic intermediates of glycolysis (glucose-6-phosphate-G6P and pyruvate). The expression of Glut1 increased in each tissue evaluated after WSSV infection, while HK, PFK and PK gene expression and enzyme activities increased in a tissue-specific manner. G6PDH activity increased during WSSV infection, and its substrate G6P decreased, while PDH activity decreased and its substrate pyruvate increased. Silencing of HIF-1α blocked the WSSV-induced Glut1 and glycolytic genes upregulation and enzyme activity in a tissue-specific manner. We conclude that HIF-1 regulates the WSSV-induced glycolysis through induction of glycolytic genes contributing to glucose metabolism in tissues of infected shrimp. Also, the inhibition, and activation of regulatory genes are likely to decrease the availability of the raw materials essential for WSSV replication and increase oxidative metabolism. |
| Databáze: | OpenAIRE |
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