Epidemiology of resistance and phenotypic characterization of carbapenem resistance mechanisms in Klebsiella pneumoniae isolates at Sahloul University Hospital-Sousse, Tunisia
| Autor: | Chrifa Chaouch, Olfa Bouallegue, Nedia Jaidane, Wejdene Mansour, Aziza Messaoudi, Noureddine Boujaâfar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Carbapenem
biology medicine.drug_class Klebsiella pneumoniae business.industry Cephalosporin Antibiotics General Medicine Drug resistance Klebsiella pneumoniae epidemiology ESBL carbapenemase phenotypic screening biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Microbiology Intensive care polycyclic compounds medicine Colistin bacteria Temocillin business medicine.drug |
| Zdroj: | African Health Sciences; Vol 19, No 2 (2019); 2008-2020 |
| ISSN: | 1680-6905 |
| DOI: | 10.4314/ahs.v19i2.24 |
| Popis: | Objective: To assess the prevalence of ESBL producing and carbapenem resistant Klebsiella pneumoniae isolated from in-come and out-come patients at Sahloul-university hospital. Methods: A retrospective study over a 3 years period (January 2012 and December 2014) focused on 2160 strains of Klebsiella pneumoniae. Statistical analysis was carried out using SPSS program. ESBL detection was performed using a double disc diffusion method and carbapenemase detection was realized by Rosco-Disk kit. Results: A total of 2160 Klebsiella pneumoniae strains were isolated during the period of the study, 26.2% (n=566) were ESBL-producers and 15.8% (n=342) showed resistance to carbapenem. The wards most affected by these strains were basically urology and intensive care units. Eighty four percent of studied strains (203/241) were resistant to temocillin, which correlate with the production of a class D (OXA-48-like) carbapenemase and 7% (17/241) showed sensitivity to EDTA and dipicolinic acid, which indicate the production of metallo-enzyme. The rate of resistance to colistin remains low. Conclusion: Resistance of Enterobacteriaceae, including K. pneumoniae, to third generation cephalosporins (3rd GC) and carbapenem through the mechanism of ESBL and carbapenemases production is becoming increasingly worrying. This suggests a more rational use of antibiotics, as well as the rigorous application of hygiene measurement. Keywords: Klebsiella pneumoniae, epidemiology, ESBL, carbapenemase, phenotypic screening. |
| Databáze: | OpenAIRE |
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