Fabrication of Electrochemical Model Influenza A Virus Biosensor Based on the Measurements of Neuroaminidase Enzyme Activity
| Autor: | Mohamed F. Diouani, Yudum Tepeli, Ülkü Anik |
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| Přispěvatelé: | Mugla Sitki Kocman University, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Université de Tunis El Manar (UTM), The grant from The Technical and Scientific Council of Turkey (TUBİTAK) Project No. 114Z654 is gratefully acknowledged., MÜ, Fen Fakültesi, Kimya Bölümü, Anık, Ülkü, Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Université Tunis El Manar ( UTM ) |
| Rok vydání: | 2016 |
| Předmět: |
alpha-2-HS-Glycoprotein
MESH: Neuraminidase/metabolism [SDV]Life Sciences [q-bio] viruses Neuraminidase Biosensing Techniques Peptides and proteins 02 engineering and technology medicine.disease_cause [ CHIM ] Chemical Sciences Models Biological 01 natural sciences MESH: Dielectric Spectroscopy Virus Analytical Chemistry Peanut Agglutinin Influenza A virus medicine MESH: Protein Binding [CHIM]Chemical Sciences Electrodes MESH: Influenza A virus/enzymology chemistry.chemical_classification Host cell surface [ SDV ] Life Sciences [q-bio] biology MESH: Peanut Agglutinin/metabolism 010401 analytical chemistry MESH: Models Biological MESH: Electrodes 021001 nanoscience & nanotechnology Fetuin Enzyme assay 0104 chemical sciences 3. Good health Dielectric spectroscopy Enzyme MESH: Biosensing Techniques chemistry Biochemistry Dielectric Spectroscopy MESH: alpha-2-HS-Glycoprotein/metabolism biology.protein 0210 nano-technology Biosensor Protein Binding |
| Zdroj: | Analytical Chemistry Analytical Chemistry, American Chemical Society, 2016, 88 (12), pp.6151-6153. ⟨10.1021/acs.analchem.6b01720⟩ Analytical Chemistry, American Chemical Society, 2016, 88 (12), pp.6151-6153. 〈10.1021/acs.analchem.6b01720〉 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.6b01720 |
| Popis: | WOS: 000378470200011 PubMed ID: 27281347 Neuroaminidase (NA) enzyme is a kind of glycoprotein that is found on the influenza A virus. During infection, NA is important for the release of influenza virions from the host cell surface together with viral aggregates. It may also be involved in targeting the virus to respiratory epithelial cells. In this study, a model electrochemical influenza A viral biosensor in which receptor-binding properties have been based on NA was developed for the first time. The biosensor's working principle is based on monitoring the interactions between fetuin A and NA enzyme. The assay was monitored step by step by using electrochemical impedance spectroscopy. Technical and Scientific Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [114Z654] The grant from The Technical and Scientific Council of Turkey (TUBITAK) Project No. 114Z654 is gratefully acknowledged. |
| Databáze: | OpenAIRE |
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