Cepharanthine as a Potential Novel Tumor-Regional Therapy in Treating Cutaneous Melanoma: Altering the Expression of Cathepsin B, Tumor Suppressor Genes and Autophagy-Related Proteins
| Autor: | Yunfen Huang, Wei Lai, Yufang Liu, Yue Zheng, Yang Xie, Mengran Peng, Qingfang Xu, Yao Lin |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
skin autophagy Histology medicine.medical_treatment cathepsin B lcsh:Biotechnology Biomedical Engineering Bioengineering Human skin Cathepsin B Targeted therapy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:TP248.13-248.65 cepharanthine Cepharanthine melanoma Medicine Original Research treatment business.industry Melanoma Autophagy Bioengineering and Biotechnology medicine.disease Radiation therapy 030104 developmental biology chemistry 030220 oncology & carcinogenesis Cutaneous melanoma Cancer research business Biotechnology |
| Zdroj: | Frontiers in Bioengineering and Biotechnology, Vol 8 (2020) Frontiers in Bioengineering and Biotechnology |
| ISSN: | 2296-4185 |
| Popis: | The incidence of primary cutaneous melanoma continues to increase annually and is one of the most aggressive malignancies in humans and need to develop more novel non-surgical therapies. Autophagy and cathepsin B targeted therapy was reported to improve melanoma treatment. Cepharanthine (CEP), a natural alkaloid extracted from the genus Cephalophyllum has been reported to have the function of inhibiting cancers. We found that CEP inhibited human primary cutaneous melanoma cells viability and proliferation in 24 h in vitro, and topical application or intra-tumoral injection of CEP decreased the growth of cutaneous melanoma in mice within 4 weeks. CEP preparations below 50% concentration did not induce skin irritation and allergy reaction on human skin in vivo. Primary cutaneous melanoma cells incubated with CEP, the expression of cathepsin B was decreased and the LC3-I and LC3-II expression changed in a dose-dependent manner, while p53, p21Cip1p, and p16Inka gene expression was up-regulated. We demonstrated the effects of CEP as a novel tumor-regional therapy for cutaneous melanoma and provided a preliminary research basis for future clinical treatment researches and the exploration of integrated treatments with systemic therapy, radiotherapy, and surgery for human primary cutaneous melanoma. |
| Databáze: | OpenAIRE |
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