Structural modification ofEscherichia colilipid A by myristoyltransferase gene fromKlebsiella pneumoniae
| Autor: | Takehiro Sugawara, Sakura Onoue, Chiho Taniguchi, Kazuyoshi Kawahara |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Klebsiella
Lipopolysaccharide Klebsiella pneumoniae Immunology Mutant Myristic acid medicine.disease_cause Microbiology Lipid A 03 medical and health sciences chemistry.chemical_compound Transformation Genetic Virology Escherichia coli medicine 030304 developmental biology 0303 health sciences biology Interleukin-6 030306 microbiology Fatty Acids Lauric Acids biology.organism_classification Lauric acid chemistry Biochemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization lipids (amino acids peptides and proteins) Myristic Acids |
| Zdroj: | Microbiology and Immunology. |
| ISSN: | 1348-0421 0385-5600 |
| Popis: | Lipid A in lipopolysaccharide (LPS) of Escherichia coli mutant strains was modified by the introduction of myristoyltransferase gene cloned from Klebsiella pneumoniae. When the gene was introduced into the mutant having lipid A containing only 3-hydroxymyristic acids, it produced lipid A with two additional myristic acids (C14:0 ). When the same gene was introduced into the mutant with pentaacylated lipid A containing one lauric acid (C12:0 ), C12:0 was replaced by C14:0 . IL-6-inducing activity of LPS with modified lipid A structure suggested that C12:0 in lipid A could be replaced by C14:0 without changing the immunostimulating activity. |
| Databáze: | OpenAIRE |
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