Thermospermine catabolism increases Arabidopsis thaliana resistance to Pseudomonas viridiflava
| Autor: | Francisco Vera Sirera, Oscar Adolfo Ruiz, María Elisa Gonzalez, Fernando Luis Pieckenstain, José Luis Rambla, Miguel A. Blázquez, Juan Carbonell, María Marina |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
defence
Arabidopsis thaliana Physiology ACAULIS5 polyamines Arabidopsis Context (language use) Plant Science Genes Plant purl.org/becyt/ford/1 [https] Ciencias Biológicas chemistry.chemical_compound Gene Expression Regulation Plant Pseudomonas Putrescine Polyamines Pseudomonas viridiflava Enzyme Inhibitors purl.org/becyt/ford/1.6 [https] Disease Resistance Plant Diseases Oxidoreductases Acting on CH-NH Group Donors biology Arabidopsis Proteins Catabolism Defence Bioquímica y Biología Molecular biology.organism_classification Polyamine oxidase Thermospermine polyamine oxidase Biochemistry chemistry thermospermine Spermine Ectopic expression Amine Oxidase (Copper-Containing) Polyamine Oxidation-Reduction CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| DOI: | 10.1093/jxb/ert012 |
| Popis: | This work investigated the roles of the tetraamine thermospermine (TSpm) by analysing its contribution to Arabidopsis basal defence against the biotrophic bacterium Pseudomonas viridiflava. The participation of polyamine oxidases (PAOs) in TSpm homeostasis and TSpm-mediated defence was also investigated. Exogenous supply of TSpm, as well as ectopic expression of the TSpm biosynthetic gene ACL5, increased Arabidopsis Col-0 resistance to P. viridiflava, while null acl5 mutants were less resistant than Col-0 plants. The above-mentioned increase in resistance was blocked by the PAO inhibitor SL-11061, thus demonstrating the participation of TSpm oxidation. Analysis of PAO genes expression in transgenic 35S::ACL5 and Col-0 plants supplied with TSpm suggests that PAO 1, 3, and 5 are the main PAOs involved in TSpm catabolism. In summary, TSpm exhibited the potential to perform defensive functions previously reported for its structural isomer Spm, and the relevance of these findings is discussed in the context of ACL5 expression and TSpm concentration in planta. Moreover, this work demonstrates that manipulation of TSpm metabolism modifies plant resistance to pathogens. The gift of SL-11061 by Dr Frydman (SLIL Biomedical Corporation, Madison, WI) is greatly appreciated. This work was supported by Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Argentina (PIP 5740, PIP 0395), Agencia Nacional de Promocion Cientifica y Tecnologica, Argentina (PICT 1119, ANPCYT), Universidad Nacional de General San Martin, Argentina (SJ10/30), Ministerio de Economia y Competitividad, Spain (BIO2011-23828), and Fundacion Carolina (postdoctoral fellowship to MM). MM, FLP, and OAR are members of the Research Career of CONICET. |
| Databáze: | OpenAIRE |
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