Nonequilibrium Spatiotemporal Sensing within Acoustically Patterned Two-Dimensional Protocell Arrays
| Autor: | Avinash J. Patil, Stephen Mann, Mei Li, Juntai Liu, Bruce W. Drinkwater, Liangfei Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Protocell
Chemical concentration Coacervate Chemistry General Chemical Engineering Substrate (chemistry) Non-equilibrium thermodynamics 02 engineering and technology General Chemistry 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Signal 0104 chemical sciences Molecule 0210 nano-technology Biological system QD1-999 Research Article |
| Zdroj: | ACS Central Science ACS Central Science, Vol 4, Iss 11, Pp 1551-1558 (2018) Tian, L, Li, M, Liu, J, Patil, A J, Drinkwater, B W & Mann, S 2018, ' Nonequilibrium Spatiotemporal Sensing within Acoustically Patterned Two-Dimensional Protocell Arrays ', ACS Central Science, vol. 4, no. 11, pp. 1551-1558 . https://doi.org/10.1021/acscentsci.8b00555 |
| ISSN: | 2374-7951 2374-7943 |
| Popis: | Acoustically trapped periodic arrays of horseradish peroxidase (HRP)-loaded poly(diallydimethylammonium chloride) / adenosine 5′-triphosphate coacervate microdroplet-based protocells exhibit a spatiotemporal biochemical response when exposed to a codiffusing mixture of substrate molecules (o-phenylenediamine (o-PD) and hydrogen peroxide (H2O2)) under nonequilibrium conditions. Unidirectional propagation of the chemical concentration gradients gives rise to time- and position-dependent fluorescence signal outputs from individual coacervate microdroplets, indicating that the organized protocell assembly can dynamically sense encoded information in the advancing reaction-diffusion front. The methodology is extended to arrays comprising spatially separated binary populations of HRP- or glucose oxidase-containing coacervate microdroplets to internally generate a H2O2 signal that chemically connects the two protocell communities via a concerted biochemical cascade reaction. Our results provide a step toward establishing a systematic approach to study dynamic interactions between organized protocell consortia and propagating reaction-diffusion gradients, and offer a new methodology for exploring the complexity of protocellular communication networks operating under nonequilibrium conditions. A combination of acoustic trapping and reaction-diffusion engineering is used to develop synthetic protocell communities capable of spatiotemporal sensing under nonequilibrium conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|