Identification of potential post-ethylene events in the signaling cascade induced by stimuli of bud dormancy release in grapevine
| Autor: | Zhaowan Shi, Michal Sharabi-Schwager, Tamar Halaly-Basha, Xuequn Pang, Etti Or, David W. Galbraith, Chen Wang, Ron Ophir, Chuanlin Zheng |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Ethylene Plant Science Biology 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Tobacco Genetics medicine Vitis Hypoxia Sodium Azide Plant Proteins Catabolism Protein Stability Autophagy Cell Biology Meristem Ethylenes Plant Dormancy Plants Genetically Modified Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Cyanamide Dormancy Seasons Nucleus Function (biology) 010606 plant biology & botany Signal Transduction |
| Zdroj: | The Plant journal : for cell and molecular biologyREFERENCES. 104(5) |
| ISSN: | 1365-313X |
| Popis: | Ethylene signaling appears critical for grape bud dormancy release. We therefore focused on identification and characterization of potential downstream targets and events, assuming that they participate in the regulation of dormancy release. Because ethylene responding factors (ERF) are natural candidates for targets of ethylene signaling, we initially characterized the behavior of two VvERF-VIIs, which we identified within a gene set induced by dormancy release stimuli. As expected, these VvERF-VIIs are localized within the nucleus, and are stabilized upon decreases in oxygen availability within the dormant buds. Less expected, the proteins are also stabilized upon hydrogen cyanamide (HC) application under normoxic conditions, and their levels peak at deepest dormancy under vineyard conditions. We proceeded to catalog the response of all bud-expressed ERFs, and identified additional ERFs that respond similarly to ethylene, HC, azide and hypoxia. We also identified a core set of genes that are similarly affected by treatment with ethylene and with various dormancy release stimuli. Interestingly, the functional annotations of this core set center around response to energy crisis and renewal of energy resources via autophagy-mediated catabolism. Because ERF-VIIs are stabilized under energy shortage and reshape cell metabolism to allow energy regeneration, we propose that: (i) the availability of VvERF-VIIs is a consequence of an energy crisis within the bud; (ii) VvERF-VIIs function as part of an energy-regenerating mechanism, which activates anaerobic metabolism and autophagy-mediated macromolecule catabolism; and (iii) activation of catabolism serves as the mandatory switch and the driving force for activation of the growth-inhibited meristem during bud-break. |
| Databáze: | OpenAIRE |
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