Structure of the genome of moloney murine leukemia virus: a terminally redundant sequence
| Autor: | Allan M. Maxam, John M. Coffin, William A. Haseltine, Thomas C. Hageman |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Gel electrophoresis
Base Sequence Genes Viral biology Oligonucleotide Oligonucleotides Nucleic acid sequence Nucleic Acid Hybridization RNA biology.organism_classification Genome Molecular biology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Nucleic acid thermodynamics Ribonucleases chemistry DNA Viral Murine leukemia virus RNA Viral Moloney murine leukemia virus DNA |
| Zdroj: | Cell. 13:761-773 |
| ISSN: | 0092-8674 |
| Popis: | The genome of the Moloney strain of murine leukemia virus (Mo-MuLV) has been analyzed by digestion with ribonuclease T1 and separation of the digestion products by two-dimensional gel electrophoresis. Thirty large oligonucleotides isolated from such a fingerprint have been characterized. One of these oligonucleotides (number 21) was found to be present in twice the molar yield of the rest. The 30 oligonucleotides were mapped on the genome by determining their yields in various size classes of 3' terminal fragments of Mo-MuLV RNA. The physical map obtained in this way suggested that oligonucletoide 21 was present very near the 3' end of the geome as well as in another location near or at the 5' end. The genome structure suggested by these results was confirmed by analyzing oligonucleotides in Mo-Mulv RNA complementary to strong stop DNA, which is shown to be a copy of the 5' terminal 134 nucleotides of the MoMuLV genome. Some of the oligonucleotides in the RNA protected from RNAase digestion by hybridization to this DNA, including oligonucleotide 21, were present near both the 3' and 5' ends. Comparison of these with the nucleotide sequence of strong stop DNA shows that there is a terminal redundancy of 49-60 nucleotides in the Mo-MuLV genome RNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|